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Examination of the medicinal profile of cedrela toona
(UMT, Lhr, 2015) RUQAIYA SHAHID
The present study was conducted to investigate the medical significance of constituents present in cedrela toona plant and importance of this medicinal plant. The chromatographic behaviour was determined by using thin layer chromatography technique. The antibacterialactivity was tested against 3 bacterial strains. The plants belonging to family meliacae has been used for various purposes especially in the formulation of medicines. Cedrela toona (C.toona) is a forest tree of family meliacae that grow throughout southern pacific. Its common name is cedar or Queen's land red cedar. It is also known as Indian mahogany. This plant show excellent growth and exhibit various activities for the treatment of certain diseases. Cedrela toona has significant antibacterial activity & shows cytotoxicity. Studies showed that C. toona exhibit antibacterial activity. The plant was extracted using methanol solvent. The tests used to study antibacterial activity was agar well diffusion method. The test was conducted on gram positive & gram negative bacteria. Various results were concluded showing the significant antibacterial activity of this plant. The Cedrela toona plant constituents were identified the plant showed significant antibacterial activity against bacterial strain. By thin layer chromatography different class of compounds were identified in the extract. Bacterial strains E coli,Bacillus thuringiensis and Staphylococcus aureus are common diseases causing bacteria in human. No activity of C. toona plant has been reported to investigate the harmful effects of these microorganisms.The present study revealed the significant antibacterial effects against these microorganisms
Synthesis, characterization, and antimicrobial properties of Mn-doped ZnO nanoparticles
(Umt, Lahore, 2015) Muhammad Waqas Bashir
In this study, synthesis, characterization and antimicrobial properties of pure and Mn-doped ZnO nanoparticles have been investigated. Undoped and Mn-doped ZnO nanoparticles were prepared by using Co-precipitation method. The synthesized Mn-doped ZnO nanoparticles were characterized by XRD, SEM, EDX, and UV-Vis spectroscopy was used to measure the band gap energy. The antimicrobial activity of Mn-doped ZnO nanoparticles was carried out by using agar diffusion method. Formation of nanoparticles was confirmed by XRD technique. XRD analysis showed hexagonal wurtzite structure of Mn doped and undoped ZnO nanoparticles. The average grain size for undoped ZnO nanoparticles was 16.72nm, and the size of Mn-doped ZnO nanoparticles was 6.14nm synthesized by co-precipitation method with different chemicals. Surface morphology and particle size of undoped and Mn-doped ZnO nanoparticles were confirmed by SEM analysis. EDX analysis was used to confirm the presence of Mn in both samples prepared by co-precipitation method. The band gap energy for Mn-doped ZnO nanoparticles was found to be 3.585eV and 2.737eV for method 1 and method 2, respectively.The antimicrobial property of Mn-doped ZnO nanoparticles against E.coli and B.subtilis were investigated by agar diffusion assay. The antimicrobial test results were good for method 2 rather than method 1. The antimicrobial results were also showed that as the concentration of Mn-doped ZnO nanoparticles was increased; the growth of inhibition zones was also increased.
Development of the clay reinforced bioceramics of gelatinsodium alginate composite scaffold for potential tissue engineering applications
(UMT, Lhr, 2015) ASIF FIAZ
A series of gelatin/sodium alginate/hydroxyapatite composite scaffolds has been prepared by using the phase separation method. The prepared spongy materials were freeze dried and subjected to dissolution test using the Ringer’s solution, which resulted in 70% dissolution and 30% retention of the materials. The porosity of the prepared materials were calculated through density measurement method. The porosity was found 82% with 150 µm pore size on average. Mechanical properties of the prepared scaffolds were determined using Instron 4502 mechanical tester. The properties like high porosity, good mechanical strength suggest that such sponges can be potentially utilized in bone tissue engineering.
Cu-doped Zno nanoparticles:
(Umt, Lahore, 2015) Shaista Jabin
In the present study, synthesis, characterization and antibacterial properties of copper doped zinc oxide nanoparticles have been investigated. Pure and copper doped ZnO nanoparticles have been synthesized by using co-precipitation method. The synthesized copper doped ZnO nanoparticles were characterized by spectroscopic and analytical techniques such as XRD, SEM, EDAX and UV-Vis spectroscopy. The antibacterial activity of copper doped ZnO nanoparticles was carried out based on the agar diffusion method. From the analysis of XRD the formation of nanoparticles was confirmed. XRD measurements show that the prepared nanoparticles have different microstructure without changing the hexagonal wurzite structure. The average grain size of undoped and copper doped ZnO nanoparticles was calculated from the XRD analysis. The calculated grain size of undoped ZnO sample was 16.72 nm and that of Cu doped ZnO samples was 17.49 nm and 20.73nm for method 1 and method 2 samples, respectively. SEM analysis confirms the surface morphology and particle size of the synthesized nanoparticles. EDAX analysis confirms the presence and amount of copper and other impurities in the sample. UV-Vis spectroscopic studies measure the amount of band gap energy. The band gap energy of Cu doped ZnO samples was 3.378eV and 3.36eV for method 1 and method 2, respectively.The antibacterial properties of copper doped zinc oxide nanoparticles against two bacteria (E.coli and B.subtilis) were investigated by using agar diffusion method. The antibacterial test results reveal that as the concentration of copper doped ZnO nanoparticles was increased, the growth of inhibition zone also increases. This reveals that Cu doped ZnO nanoparticles show antibacterial activity against these two bacteria i.e. E.coli and B.subtilis.
Anticancer activity of ethyl acetate extract of tanacetum parthenium
(UMT, Lhr, 2015) SHAHID YOUNIS
The aim of research was to evaluated the proximate analysis, phytochemical screening, antibacterial activity, anticancer activity of the Tanacetum parthenum plant. The proximate analysis was used to characterize the plant on the basis of different percentage of different plants contents and so percentage of moisture, fiber, and ash, fat was determined. The phytochemical screening of plant indicated that chemical compound i.e. flavonoids, tannins, reducing sugar, starch, coumarins was indicated in it and due to the presence of different active chemical compounds it was used against bacteria. The plant extract has shown positive inhibition effect against bacteria and it restricts the growth of bacteria. The prepared solution of ethyl acetate and methanol were applied to check their effect on the growth of cancerous HeLa cell line. The neutral red dye assay was used to evaluated the percentage viability of the cells after treated with ethyl acetate and methanol extract. The treated cells were studied at 540 nm to check their absorbance. The 100% absorbance of the control group was compared with absorbance of treated experimental cancer cells. The decrease in absorbance means decrease in cancerous cell after treated with extracts. The concentration of acetate extract was found to be 128 folds dilution (0.004 µg) at which the number of cancer cells reduced to 50%. The half inhibitory concentration of the methanolic extract was greater than 128 folds dilution at which viability of the cells were reduced to 50% so, methanol extract is more potent inhibitor for cancerous activity of the cells.
Synthesis and characterization of metal ferrites and study of their magnetic properties in polymer thin films
(UMT, Lhr, 2015) ABDUL SATTAR
Nanoparticles of Nickel ferrites have been prepared using the modified co-precipitation technique; the methodology involved the controlled mixing of Nickel chloride 0.2M solution and Ferric chloride 0.4M solution in the ratio of 1:2 respectively. The precipitates were dried at 105oC for 24h, annealed at 600oC for one hour and characterized using SEM and XRD techniques. The SEM and XRD results showed that prepared particles were in nano range. Prepared material having good surface morphology was incorporated into starch grafted methylmeth acrylate copolymer by in-situ polymerization method. The nano composite prepared in this way was characterized by SEM, FTIR and VSM for the magnetic properties. The results of these techniques proved the usefulness of the material as biodegradable thin films having controllable magnetic properties for potential use as magnetic strips.
Compositional analysis of non polar extract of melia azedarach
(Umt, Lahore, 2015) Rabia Habib
Medicinal plants are widely used by the traditional practitioners to cure different diseases due to their world-wide availability and fewer side effects. Melia azedarach L. belongs to the family Meliaceae which is a family of highly significant medicinal plants. Traditionally, different parts such as leaf, flower, seed, fruit, and young branches of M. azedarach have been used for the treatment of malaria, diabetes, purgative, cough, skin disease, and many more. Experiment and clinical studies prove that it has antimicrobial, antioxidant, anti-inflammatory, cardioprotective, anticancer, analgestic, antiulcer, antipyretic, antiplasmodial and male contraceptive properties. Thus the aim of this study was to explore the chemical profile of non-polar extract of M. azedarach leaves through GC-MS analysis, as well as to check the antimicrobial activity of this plant against different bacterial strain by using agar diffusion method. The identification of phytochemical compounds was carried out by comparing molecular ion peak, base peak, and fragmentation pattern. GC-MS analysis of hexane extract of M. azedarach showed a highly complex profile, containing ketones, ethers, fatty acid derivatives, methyl esters, 1,3-dipalmitate, 7,8-dihydrocarpesterol, and 2-Undecanol. The results of antibacterial activity of hexane extract showed that M. azedarach possessed mild resistance against the bacterial infection. This study also indicated that hexane extract of M. azedarach was more potent against the Gram-positive than the Gram-negative bacteria. This study will be useful to explore the pharmacological and biosynthetic activity of plants further.
Fabrication and characterization of cadmium sulfide nanowires on aluminum oxide template
(Umt, Lahore, 2015) Malik Imran Afzal
Cadmium supplied nanowires have unique electrical and optical properties and applications. To obtain cadmium supplied nanowires with regular and good aspect ratio, they can be synthesized by template synthesis method. Porous anodized aluminium oxide is most promising template with regular hexagonal shapes. Their aspect ratio can be controlled by controlling the pores’ depth and diameter which greatly depend on anodization voltage and temperature of the electrolyte. In this research, high purity aluminium was used to prepare nanotemplates at 5-6°C in 1M phosphoric acid and cadmium supplied was deposited electrochemically using a co-solution of thiourea, cadmium acetate and ammonium acetate. pH was maintained at 11 in heat bath at 75°C with the help of aqueous ammonia solution. Both porous anodized alumina and cadmium supplied Nanowires were characterized suing SEM. A good quality Nanowires were obtained in bunches with reasonably high aspect ratio.
Synthesis, characterization and antibacterial activities of iron oxide nanoparticles
(UMT, Lhr, 2015) MUHAMMAD UMAIR
The importance of nanoparticles is increasing day by day due to their potential utility in the fields if biomedicines, photo catalysis and in nano composite materials. Iro oxide nanoparticles can be effectively used in biomedical field for drug delivery, MRI and magnetic hj^erthermia. In this study, synthesis, characterization, and antimicrobial properties of iron oxide nanoparticles was investigated. Iron oxide nanoparticles were synthesized by co-precipitation method with different routes and chemicals. Synthesized iron oxide nanoparticles were characterized by X-ray Diffraction, Scanning Electron Microscope, Energy Disperdive X-ray spectroscopy and UV-Vis Spectroscopy and antibacterial activity of synthesized nanoparticles was also measured by determining the inliibition zones against Gram (+) and Gram (-) bacteria. Gram (+) bacteria species was Bacillus subtilis and Gran (-) bacteria species was Escherichia colt. Antibacterial activity was measured by using agar diffusion method. Formation of nanoparticles was confirmed by XRD technique. XRD analysis showed that the average grain size of the synthesized iron oxide nanoparticles was 19.11nm, 18.16nm, and 14.37nm for method-1, method-2 and method-3 respectively. Surface morphology and particle size of iron oxide nanoparticles were confirmed by SEM analysis. EDX analysis was used to study the samples and this analysis has confirmed the presence of Fe and O in all samples prepared by co-precipitation method with different routes chemicals. The nanoparticles which were synthesized by three methods marked as U-3, U-4 and U-5 for method-1, method-2 and method-3 respectively. All the three samples have grain size in nanometer range as; 19.11nm for U-3, 18.16nm for U-4 and 14.37nm for U-5. Smallest grain size from all three samples was 14.37nm of sample U-5 was further subjected for band gap analysis. Theband gap for nanoparticles was measured as 1.46eV for sample U-5. Sample U-5 was also used for studying antibacterial activity against the Gram (+) and Gram (-) bacterial cultures and proved as an excellent antibacterial agent.
Hypoglycemic activity of pistachia khinjuk
(UMT, Lhr, 2015) SAIMA TAJ
Diabetes is spreading all over the world day by day. There are many ways to treat diabetes mellitus which includes synthetic drugs, Homeopathic medicine or Unani Medicine etc. These Medicines have many side effects. Natural products include herbs, shrubs and large plants which cure diabetes mellitus and prove excellent hypoglycemic activity. These herbal medicines have no side effects. Almost all the species of family Anacardiaceae show hypoglycemic activity and used from centuries to cure diabetes. Many pistachio species show hypoglycemic activity and have a very long history of herbal remedies. The present study evaluates the hypoglycemic effect of Pistachia khinjuk. In this study, hypoglycemic effect is checked from the extract and wax of Pistachia khinjuk individually. Six groups of Swiss albino mice were made for wax and six for extract (80:20 Methanol: water) of Pistachia khinjuk separately and each group contains six albino mice. All the mice were injected alloxan monohydrate except normal group of wax and extract. Group 1 was treated as normal group and receives no treatment, group 2 receive 5mg/kg of glibenclamide after alloxan monohydrate induction, group 3 receive no treatment after alloxan monohydrate induction, group 4 and 5 receive 500 and 250mg/kg of Pistachia khinjuk extract while group 6 receives 500mg/kg Pistachia khinjuk wax after alloxan monohydrate treatment. Almost all the mice for wax (Pistachia khinjuk) of group 4, 5 and 6 show gradual increase in their diabetes while all the mice for extract (Pistachia khinjuk) of group 4, 5 and 6 show hypoglycemic activity and decreases blood glucose level. Present study indicated the hypoglycemic effect of Pistachia khinjuk for extract but indicates opposite results for wax of Pistachia khinjuk. There is a clear difference of increased blood glucose level for wax of Pistachia khinjuk. There may be many factors behind this activity which needs more research on it by detecting and analyzing specific compound which cause this effect. The methanolic extract due to phenolic constituents proves to be excellent antidiabetic medicine.
Characterization of sunflower oil for its Oleic Acid content
(Umt, Lahore, 2015) Mehwish Shahzadi
Sunflower is cultivated all over the world not only as an ornament plant but also for the purpose of getting oil. It is the third most cultivated plant in the history because its oil considered best for health.The present study deals with the analysis of sunflower oil sample which was obtained from local market. The physicochemical properties of the oil were determined which included saponification value, acid value and ester value. Results showed that saponification value of the oil was 191.675, acid value was 0.94 and ester value to be 190.735 for the sample under observation. GC-MS analysis of sunflower oil was carried out to check its composition. Oleic acid was determined with linoleic acid and isopropyl palmitate. Other compounds detected were p-toluylic acid, pregn-4-en-18-oic acid, 11-(acetyloxy)-6,7-epoxy-9,20-dihydroxy-3-one-, gamma.-lactone, 1,2-benzenedicarboxylic acid, benzoic acid, 2,4,6-trimethyl-, 2,4,6-trimethylphenyl ester and linoleic acid.
Estimation and stability studies of amoxicillin trihydrate in the capsule dosage form
(UMT, Lhr, 2015) MAAZ ASIM
A simple, rapid and time saving method for the validation of Amoxillin Trihydrate in capsule dosage form has been developed. Recovery studies was achieved by HPLC for this chromatographic separation was achieved by phenomenex [250x4.6 mm with octadecylisily silica gel for chromatograph (5µm)] column on binary mode and mobile phase (A) contain 6.8g of potassium dihydrogen phosphate buffer (adjust to pH 5.0 by sodium hydroxide solution) containing Acetonitrile with ratio of 99:1 and mobile phase (B) containing Buffer and Acetonitrile with ratio of 80:20. The flow rate was 1 ml/min with 8% concentration of mobile phase (B) and was monitored at 254 nm. Retention time was found to be near 7.1 for Amoxicillin Trihydrate. The regression value obtained was 0.9999 with 3.0011 Limit of Detection and 9.0943 Limit of Quantization. Residual solvents were also validated for AMT by using GC-FID and observed that there is no presence of any solvent in it. Other parameters like FTIR, pH, Optical rotation and Moisture contents were also performed for further analysis and all results were satisfactory and was meeting the require values. The projected methods are accurate, exact, selective and rapid for the estimation of Amoxicillin Trihydrate in pharmaceutical dosage forms.
Synthesis and biological screening of some Azomethine derivatives of 2,4-Dimethylcarbolic Acid
(Umt, Lahore, 2015) Ata Ur Rehman
The molecules comprising azomethine group are famous to possess biological activities. In the present work, the synthesis of N'-Substituted benzylidene-2-(2,4-dimethylphenoxy) acetohydrazide (5a-d) has been performed using 2,4-Dimethylphenol (1) as precursor. The molecule 1, was transformed into ethyl 2-(2,4-dimethylphenoxy)acetate (2) on refluxing with ethyl 2-bromoacetate in ethanol medium in the presence of base KOH. Ethyl ester 2, was refluxed with hydrated hydrazine (80%) in ethanol medium to produce 2-(2,4-dimethylphenoxy) acetohydrazide (3). The target molecules 5a-d, were manufactured by stirring 3 with phenyl/aryl carboxaldehyde (4a-d) in methanol medium in the presence of glacial acetic acid. The synthesized molecules were characterized by physical spectral data and estimated for antibacterial and anti-enzymatic activities.OVERALL SCHEME FOR SYNTHESIS OF AZOMETHINE DERIVATINES Scheme 1: Outline for synthesis of N'-Substitutedbenzylidene-2-(2,4-dimethylphenoxy) acetohydrazide (5a-d); Reagents and conditions: (A) Ethyl 2-bromoacetate/EtOH/ KOH/Reflux for 6 hours (B) 80% Hydrated hydrazine/EtOH/Reflux for 4 hours (C) Phenyl/aryl carboxaldehydes (4a-d)/MeOH/Glacial acetic acid/Stir for 3-4 hours.
Development of the gelatin/sodium alginate composite scaffold for potential tissue engineering application
(UMT, Lhr, 2015) AHTSHAM ASHRAF
A series of gelatin/sodium alginate composite scaffolds has been prepared by using the phase separation method. The prepared spongy materials were freeze dried and subjected to dissolution test using the Ringer’s solution, which resulted in 70% dissolution and 30% retention of the materials. The porosity of the prepared materials were calculated through density measurement method. The porosity was found 82% with 150 µm pore size on average. Mechanical properties of the prepared scaffolds were determined using tensiometer. The properties like high porosity, good mechanical strength suggest that such sponges can be potentially utilized in bone tissue engineering.
Synthesis, characterization and biological activities of co-doped SNO2 nanoparticles
(UMT, Lhr, 2015) MUHAMMAD AZAM QAMAR
Synthesis and applications of nanoparticles have got great importance in different fields. Cobalt-doped SnO2 nanoparticles were prepared by using a simple synthesis method, and their characterization, optical properties and antimicrobial activities were investigated. The co-precipitation technique was applied to synthesize doped, highly pure nanoparticles. The formation of the prepared nanoparticles was confirmed by applying the important characterization techniques. The used techniques were Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD) analysis, and UV-Vis. spectroscopy. The average size of the nanoparticles was found to be 24.86 nm. The SEM/EDX analysis data confirmed the elemental composition of Co-doped SnO2 nanoparticles. The optical properties were studied by measuring the energy band gap. The Energy band gap was found 1.477 eV for un-doped SnO2 which decreases to 1.450 eV for doped SnO2 nanoparticles. The antimicrobial activities of the synthesized nanoparticles were carried against both Gram-negative than Gram-positive bacteria. The antimicrobial activity of doped SnO2 nanoparticles was good against Gram-negative as well as against Gram-positive bacteria
Synthesis and characterization of gelatin grafted biodegradable plastic using acrylonitrile and vinyl acetate
(UMT, Lhr, 2015) WAJAD ULFAT
The backbone of gelatin was grafted with the monomers of vinyl acetate and acrylonitrile. This grafted copolymer was obtained by the employing of free radical polymerization technique with potassium per sulfate which acted as initiator in the reaction. The stirring and heating of reaction was constant at 80 oC. The polymer was characterized by the use of FTIR, TGA and DSC. FTIR proved the grafting of monomers on the backbone of the gelatin. TGA-DSC analyzed the degradation and melting of the copolymer and nanocomposite polymer. The nanocomposite polymer was prepared with the incorporation of suitable quantity of Nickel doped nanoparticles. Soil burial method was used for the study of biodegradation of this polymer and its test range is 60 days. The degradation study of prepared samples W-1 and W-2 was shown to degradation results 29.9 and 11.05 percent respectively. At the beginning of the test was observed that weight of the copolymer (W-1) was increased due to the absorption of moisture from the soil. But after 20 days the weight of polymer was decreased constantly. The W-1 sample showed the high degradation rate. The nanocomposite polymer W-2 sample showed the resistance against the biodegradation. Nanocomposite polymers were also less moisture absorber. Results showed that the grafting was a unique technique which induced the biodegradation that was not present in the synthetic polymers. The nanoparticles imparted the resistance against the microbes; therefore these nanocomposite polymers are best for the packing of food.
Synthesis, characterization, optical and antibacterial properties of Ni-DOPED ZnO Nano-particles
(UMT, Lhr, 2015) MUDASSAR SHER
The importance of nano-particles is increasing day by day due to their potential utility in biomedicines, solar cells and in nano-composite materials. Further the effect of doping to enhance the properties of nano-particles is also under research in current period all over the world. In this work, co-precipitation and solvo-thermal methods were employed and both methods successfully yield Ni-doped zinc oxide nano-particles. We also synthesized un-doped zinc oxide nano-particles via solvo-thermal method. All prepared nano-particles were characterized through X-ray diffraction studies. To validate nickel doping in zinc oxide nano-particles Energy Dispersive X-ray analysis were further employed and Energy Dispersive X-ray results confirmed desired doping in all samples. Scanning Electron Microscopy analysis was performed to study shape and morphology of all prepared nano-particles samples. The results of all above mentioned characterization methods confirmed successful preparation of Ni-doped zinc oxide nano-particles through both preparation methods. All synthesized sampled were also employed to check their antibacterial activity. In Antibacterial study, zones of inhibition have been calculated against Gram positive (Bacillus Subtilis) and Gram negative (Escherichia coli) bacterial species. Different molar concentrations of Ni-doped zinc oxide nano-particles were checked through ager well diffusion method. The results showed that antibacterial activity is directly proportional to concentration of Ni-doped zinc oxide nano-particles Furthermore Gram positive bacteria are found generally more affected with synthesized nano-particles as compared with Gram negative bacteria. The effective antibacterial activity has shown by all prepared Ni-doped zinc oxide samples favor their use as antibacterial agent. Band gap analysis was performed to check optical activity for all samples. It was noted that doped sample shown enhanced optical character when compared with un-doped samples.
Preparation and characterization of biodiesel from waste vegetable oil
(UMT, Lhr, 2015) MUHAMMAD TAWSIF
Biodiesel was synthesized from waste cooking oil by three-step method. These steps include saponification, acidification and esterification. The waste cooking oil was collected from the restaurants of Gujranwala. During saponification, molar ratio of WCO and caustic soda was reacted accordingly to a reported optimized value. Silica gel was used as dehydrating agent to absorb water from the synthesized biodiesel. The percentage yield obtained by this method was 79%. Physical and chemical properties like viscosity, density, flash point, cloud point, saponification value, acid value and ester value were examined to analyze the quantity of biodiesel.
Development of HPLC method for cefixime estimation in the capsule dosage form
(UMT, Lhr, 2015) AYESHA NAVEED
A precise, accurate, cost effective and rapid High Performance Liquid Chromatographic (HPLC) method for the estimation of Cefixime Trihydrate in capsule dosage form is put forward and also validated. The chromatographic system consisted of phenomenex [250x4.6 mm with octadecylisily silica gel for chromatography (5µm)] column on binary mode and mobile phase comprising of Acetonitrile and tetra butyl ammonium hydroxide solution (buffer solution) with ratio of 350:750, respectively. The pH=6.5 was regulated with phosphoric acid at a flow rate of 1mL/min and the retention time was almost 20 minutes. The regression value obtained was 1.0000 with 0.3340 µg/mL Limit of Detection and 1.012 µg/mL Limit of Quantization. Through this method related substances were also determined with precise results which were less than 1%. All the parameters like FTIR, UV-VIS, pH, and Moisture contents were found to be satisfactory for the analyzed sample. This method is precise, accurate, cost and time effective for the estimation of Cefixime Trihydrate in capsule dosage forms.
Development and validation of analytical method of silymarin by rp-hplc with uv detector
(UMT, Lhr, 2015) SOHAIL ASLAM
Milk Thistle (Silybum marianum) is a wild, herbaceous plant. Generally the extract of Silybum marianum is expressed as total Silymarin. Silymarin is composed of flavonolignans, silybin, silydianin and silychristin. Out of these compounds, silybin is considered the most vital as it plays an important role for the treatment of liver disorder. Silymarin is very important natural drug which is widely used for the treatment of variety of diseases, especially for hepatic disorders. A novel, reliable and a sensitive method to determine the amount of Silymarin was developed by using reversed phased HPLC method with isocratic solvent delivery. Silymarin was separated using Hypersil ODS C18 column maintaining temperature at 40°C in the column oven and detector was set at wavelength 288 nm with mobile phase in a ratio Methanol:Water:Phosphoric acid (46:54:0.1 v/v/v). The silymarin was tested with respect to silybin which is the most vital and pharmacological active substance. For this purpose the retention time of the silybin among all flavonolignans were determined by injecting silybin standard. Then the silymarin standard and silymarin raw material sample were injected and successfully determined the assay of silymarin. The developed method was then validated as per ICH guidelines. Linearity, accuracy, precision and robustness are the key parameters used to validate the method. This method could easily be used for the determination of silymarin for routine analysis of raw material to maintain its quality and to check the efficacy of the incoming consignments of raw material.

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