Synthesis, characterization and antibacterial activities of iron oxide nanoparticles
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Date
2015
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Publisher
UMT, Lhr
Abstract
The importance of nanoparticles is increasing day by day due to their potential utility in the fields if biomedicines, photo catalysis and in nano composite materials. Iro oxide nanoparticles can be effectively used in biomedical field for drug delivery, MRI and magnetic hj^erthermia. In this study, synthesis, characterization, and
antimicrobial properties of iron oxide nanoparticles was investigated. Iron oxide nanoparticles were synthesized by co-precipitation method with different routes and
chemicals. Synthesized iron oxide nanoparticles were characterized by X-ray Diffraction, Scanning Electron Microscope, Energy Disperdive X-ray spectroscopy
and UV-Vis Spectroscopy and antibacterial activity of synthesized nanoparticles was also measured by determining the inliibition zones against Gram (+) and Gram (-)
bacteria. Gram (+) bacteria species was Bacillus subtilis and Gran (-) bacteria species was Escherichia colt. Antibacterial activity was measured by using agar diffusion
method. Formation of nanoparticles was confirmed by XRD technique. XRD analysis showed that the average grain size of the synthesized iron oxide nanoparticles was 19.11nm, 18.16nm, and 14.37nm for method-1, method-2 and method-3 respectively. Surface morphology and particle size of iron oxide nanoparticles were confirmed by SEM analysis. EDX analysis was used to study the samples and this analysis has confirmed the presence of Fe and O in all samples prepared by co-precipitation method with different routes chemicals. The nanoparticles which were synthesized by three methods marked as U-3, U-4 and U-5 for method-1, method-2 and method-3 respectively. All the three samples have grain size in nanometer range as; 19.11nm for U-3, 18.16nm for U-4 and 14.37nm for U-5. Smallest grain size from all three
samples was 14.37nm of sample U-5 was further subjected for band gap analysis. Theband gap for nanoparticles was measured as 1.46eV for sample U-5. Sample U-5 was also used for studying antibacterial activity against the Gram (+) and Gram (-) bacterial cultures and proved as an excellent antibacterial agent.