Validation studies for Methamphetamine by using FTIR, GC-MS And UV-visible spectroscopy
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Date
2017
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Publisher
University of Management and Technology Lahore
Abstract
Background: Methamphetamine belongs to the class of amphetamine type stimulants. It is illicitly manufactured in clandestine laboratories. It is strong stimulant of central nervous system. Major precursors required for the manufacturing of methamphetamine includes ephedrine, pseudo-ephedrine and phenyl-2-propanone. Its manufacturing, possession, trafficking, distribution and sale have been restricted and controlled not only in Pakistan but internationally as well. Here we proposed a method for the detection and quantification of methamphetamine manufactured in Pakistan. The proposed method is quiet easy, simple, cost effective, require no complex methodology for sample preparation and its results are quiet reliable, accurate and reproducible. The detection or confirmation of methamphetamine was done by Gas Chromatography- Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR).The quantification of methamphetamine was performed by using UV-Visible Spectrophotometer and all the parameters like accuracy, precision, linearity, reproducibility, limit of detection, limit of quantification etc were validated. The linearity range was 100 µg/ml to 1000 µg/ml and the observed lambda max was 259 nm. The proposed method is easy, simple, and accurate and results are so reliable that it can be used for the case work in forensics and for drug analysis.
Methods: The presumptive color testing was used for the detection of methamphetamine. The confirmation of methamphetamine was done by Gas Chromatography- Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR) and the quantification of methamphetamine was performed by using UV-Visible Spectrophotometer.
Results: The linearity range was 100 µg/ml to 1000 µg/ml and the observed lambda max was 259 nm. The calibrators were stable for continuous 4 days. The limit of detection was 50 µg/ml and limit of quantification was 100 µg/ml. The absorbance showed by methamphetamine in UV-Visible range is due to π to π* transition. FTIR spectrum showed that sample was 88 % pure and that of GC-MS showed that sample contain 83% methamphetamine.
Description
Supervised by:Dr. Khurram Shahzad Munawar
Keywords
Methamphetamine, Pseudo-ephedrine, MS Thesis