SOP

Permanent URI for this community

https://escholar.umt.edu.pk/handle/123456789/9254

Browse

Browsing SOP by Title

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    ANTI-HYPERGLYCEMIC EFFECT OF ARGYROLOBIUM ROSEUM (Cambess.) jaub. & spach
    (UMT, Lahore, 2025) Sarah Kausar
    Diabetes mellitus is slowly poisoning disease and becomes a matter of concern from last decade because of its prevalence. In this regard a research plan designed to address the global disease. A wild herb testing against the disease with STZ model. ARGYROLOBIUM ROSEUM (CAMBESS.) Jaub. & Spach is annual wild herb grow in sandy rock places. It is commonly found in the Pakistani hilly areas in February. It is flowering and fruiting plant belongs to family papiolionaceae. Ethanolic extract prepared against the plant for in vivo testing (rats) against STZ and in vitro testing (GC MS). An ethanolic extract prepared from dried herb. Some extract again fractionated in ethyl acetate n hexane aqueous extracts for GC MS. For in vivo study take Sprague Dawely rats 4(n=5) and induced diabetes with STZ. Before that acute toxicity testing and observe no sign of toxicity. The in vivo model planned for 28 days. The standard used against is metformin. After testing all rats sacrificed and sample took for biochemical markers (HbA1c, ALT AST, APL, urea, and creatinine). Histopathological study also conducts. In results plant decrease diabetes and Beta cell neogenesis. Biochemical markers not show any significant change compare to normal. Oral glucose tolerance test poor show sustainability. Ethyl acetate extract show less hypoglycemic activity. GC MS show mostly phenols and fatty acid ester.
  • Thumbnail Image
    Item
    ANTIEPILEPTIC AND NEUROPROTECTIVE POTENTIAL OF ACACIA NILOTICA (KEKKAR)
    (UMT, Lahore, 2025) Hooria Gul
    Epilepsy remains a major global health challenge, with approximately one third of patients experiencing drug resistant seizures and enduring adverse effects from conventional antiepileptic drugs. This study evaluated the antiepileptic and neuroprotective potential of an ethanolic bark extract of Acacia nilotica (AnEth) in Balb/c mice subjected to Pentylenetetrazol (PTZ) induced kindling. Phytochemical profiling by FTIR, LC-MS/MS, and GC-MS confirmed a rich composition of polyphenols, flavonoids, tannins, and triterpenoid saponins. The extract exhibited strong antioxidant activity (88%DPPH scavenging at 10 mg/mL) and was found to be safe up to 2000 mg/kg in an acute oral toxicity assay. In the chronic PTZ model(40 mg/kg,i.p.,on alternate days for 21 days),mice were randomized into five groups(n=8).Seizure incidence,latency,duration,and modified Racine scores were recorded, alongside behavioral assessments)and body weight monitoring alone induced seizures in 87.5% of mice, with mean Racine scores rising to 3.3±1.3.Diazepam fully prevented seizures, whereas AnEth at 200 mg/kg and 400 mg/kg reduced seizure incidence to 50% and 37.5%,and mean Racine scores to 1.25±0.5 and 1.7±1.1,respectively (p<0.01 vs. PTZ). Both AnEth doses ameliorated PTZ induced weight loss and improved anxiety like behaviors. The extract’s favorable safety profile and multifaceted neuroprotective actions support its potential development as alternative therapy for refractory epilepsy. Plant extract shows promising antioxidant trends, PTZ treatment caused oxidative stress in the brain, shown by a rise in antioxidant enzymes like Catalase and SOD
  • Thumbnail Image
    Item
    EVALUATION OF ANTI-DIABETIC ACTIVITY OF ESSENTIL OIL OF ARTEMISIA MARITIMA. L IN HYPERGLYCEMIC RATS
    (UMT, Lahore, 2025) Iqra Habib
    This study investigated into the toxicity and antidiabetic properties of an essential oil that was extracted from Artemisia maritima. Notably, this research represents the first investigation into the antidiabetic effect of Artemisia maritima essential oil. The steam distillation process was used to extract the essential oil. The essential oil was analyzed by gas romatography (GC), GC/mass spectrometry (GC/MS). There were twelve compounds found in Artemisia maritima. The primary constituents of oil from Artemisia maritima were Eucalyptol, (+)-2-Bornanone, 3-cyclohexen-1-ol, 4-methyl-1-(1-methylethyl)-, (R)-, (+)-4-Carene, o-Cymene, gamma- Terpinene, Thujone, 4-Isopropyl-1methlycyclohex-2-enol, alpha-Terpineol, 2-Cyclohexen-1- one,3-methyi-6-(1-methylethyl)-, Bicyclo[7.2.0]undee-4-ene,4,11,11-trimethy-8-methylene- and Germacrene D. The exhibited biological potential of Artemisia maritima may be attributed to the active ingredients identified by the phytochemical analysis. Rats with diabetes caused by streptozotocin were given essential oil of Artemisia maritima for a period of 28 days. The rats were killed after 28 days, and heart punctures were used to obtain blood samples. Blood glucose levels as well as liver and kidney enzyme levels were assessed. These results suggest that diabetes mellitus can be managed using the AMEO.