2019

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Browsing 2019 by Author "BASMA SHAHID"

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    Green synthesis of manganese oxide nanoparticles and their applications
    (UMT, Lhr, 2019) BASMA SHAHID
    This research work includes the green synthesis of Manganese Oxide nanoparticles by using the leaves of Abutilon indicum which is found in open areas and uncultivated land. It comprises alkaloids, flavonoid, steroids, tannins, terpenoids and saponins that are responsible for the reduction and stabilization of nanoparticles. Plant extract was used for the synthesis of manganese oxide nanoparticles which act as reducing and capping agent. Different characterization techniques were used for the confirmation of synthesis and to determine the morphology and composition of nanoparticles such as, XRD, EDS and UV-Vis spectroscopy. Analysis of XRD confirmed the small size and good crystallinity of nanoparticles. It also confirmed the purity of nanoparticles because no other peaks were shown in XRD spectrum. These synthesized nanoparticles were used further to analyse their different applications. Antibacterial activity was performed using gram-positive and gram-negative bacteria (E. coli, B. bronchiseptica, S. aureus, and B. subtilis). Leflox and DMSO were used as positive and negative control, respectively. Different dilutions of nanoparticles were used and zones of inhibition were measured. Anticancer activity was also performed that confirmed that at low concentration, cell survival is high. Dye degradation activity was carried out for about 180 minutes under both sunlight and UV-lamp light for comparison of percentage degradation that confirmed that sunlight shows higher degradation as compared to UV-lamp light. Reaction was performed thrice to check the stability of nanoparticles, results reported that degradation efficiencies slightly decreases on each run. These nanoparticles were then used for the adsorption of Chromium (VI). Different concentrations of nanoparticles ranging from 0.01 to 0.05 g/ml were used with same concentration of Cr (VI). Results confirmed that by increasing the concentration, percentage adsorption increases. Then, it was also analyzed by changing the pH and kept remaining concentrations constant that confirmed that it gives good results in acidic medium.