2019

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Comparative estimation of modafinil tablet using different analytical methods
(UMT, Lhr, 2019) ARIHA ZAFAR
Simple, fast and time saving analytical methods for the comparison of Modafinil drug from two different medical brands (Local and Imported) had been tested. In this study UV spectroscopic method and High performance liquid chromatographic (HPLC) methods were used for the analytical assesment of modafinil tablets. UV absorbance (observed at 252nm) was different for different concentrations 10, 20, 30, 40, 50µg/ml). 10 % aqueous methanol was used as diluent as well as blank. In HPLC, the mobile phase was composed of buffer (6.8 g/L of potassium dihydrogen phosphate in water) and acetonitrile in (65:35) ratio. The flow rate was 1.0 ml/min and the wavelength of detector was 220nm. HPLC analysis was used to compare the area and retention time of the both modafinil containg tablets. The area and retention time from HPLC analysis of the both modafinil containing tablets were compared. Different concentrations (0.5, 1.0, 1.5, 5.0, 10, 20, 30 µg/ml) were used and a linear increase in the area and retention time of the both Modafinil containing tablets were observed. It was observed that, both the local and imported have accurate amount of active ingredient as mentioned on their box.