Hypoglycemic and free radical scavenging activity of triticum aestivum(wheat) sprout extracts on diabetic mice
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Date
2017
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University of Management and Technolog
Abstract
Triticum aestivum is traditional medicinal plant used to treat diabetes, oxidative stress, cancer, ulcer, rheumatoid arthritis, severity of rectal bleeding, thalassemia etc. The current work was planned to study the hypoglycemic, antioxidative and free radical controlling properties of ethanolic extracts of wheat sprouts Triticum aestivum. Six groups of Swiss albino mice were made and each group contains six albino mice. All the mice were injected alloxan monohydrate except normal group. Group 1 was treated as normal group and receives no treatment, group 2 receive 5mg/kg of glibenclamide after alloxan monohydrate induction, group 3 receive no treatment after alloxan monohydrate induction, group 4, 5 and 6 receive 500, 250 and 100mg/kg of wheat sprouts extract after alloxan monohydrate treatment respectively. All the mice of group 4, 5 and 6 show hypoglycemic activity and decreases blood glucose level, also increase in liver glycogen. The ethanolic extract of plant were screened for their antioxidant activity by different assays like DPPH radical scavenging assay, total antioxidant activity by phosphomolybdenum method and total phenolic content assay. Results displayed that ethnolic extract of wheat sprouts had very good antioxidant activity in DPPH assay and phosphomolybdenum method. TPC values ranging from 0.0170g/100g GAE to 0.6856g/100g GAE. There may be many factors behind this activity which needs more research on it by detecting and analyzing specific compound which cause this effect. The present results obtained from this study showed that the ethanolic extract of Triticum aestivum wheat sprouts possess significant antihyperglycemic and antioxidant activities in Swiss albino diabetic mice.
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Supervised by:Dr. Sammia Shahid
Keywords
Triticum aestivum wheat sprouts, phosphomolybdenum method and total phenolic, MS thesis