Controlled assembly of silver nano-fluid in heliotropium crispum extract: a potent anti-biofilm and bactericidal formulation.

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dc.contributor.authorNauman Khalid
dc.date.accessioned2018-10-09T06:48:33Z
dc.date.available2018-10-09T06:48:33Z
dc.date.issued2016
dc.description.abstractThe study describes the optimized method for silver nanoparticle (AgNPs) synthesis using Heliotropium crispum (HC) plant extract. Optimization of physicochemical parameters resulted in stable and rapidly assembled AgNPs. FTIR results suggest presence of plant phytochemicals that helped in the reduction, stabilization and capping of AgNPs. The assembled Ag nano-composites displayed the peak surface plasmon resonance (SPR) around 428 nm. The presence of uniquely assembled Ag-biomolecule composites, cap and stabilize nanoparticles in aqueous plant suspension. Spherical, uniform-shaped AgNPs with low poly-dispersion and average particle size of 42 nm and was determined through dynamic light scattering (DLS) and scanning election microscopy (SEM) which present robust interaction with microbes. The study also evaluates the antimicrobial and anti-biofilm properties of biologically synthesized AgNPs on clinical isolates of MRSA, Pseudomonas aeruginosa and Acinetobacter baumannii. Minimum inhibitory concentration (0.5 mg mL−1) of nanoparticles that presented bactericidal effect was made through inhibition assays on bacterial strains. The concentration which presented potent bactericidal response was then evaluated through growth inhibition in liquid medium for anti-biofilm studies at 2.0 mg mL−1. HC-Ag nanoparticles mediated anti-biofilm effects on Pseudomonas aeruginosa was revealed through SEM. Complete breakdown of biofilm’s extracellular polymeric substances resulted after incubation with AgNPs. Peptidoglycan cell wall destruction was also revealed on planktonic bacterial images after 24 h of incubation.en_US
dc.identifier.citationKhan, F., Hashmi, M. U., Khalid, N., Hayatf, M. Q., Ikram A., & Janjuaa. H. A. (2016). Controlled assembly of silver nano-fluid in heliotropium crispum extract: a potent anti-biofilm and bactericidal formulation, applied surface science, 387, 317-331. (Nauman Khalid (SFAS), JCR LISTED (IF:3:15))en_US
dc.identifier.urihttps://escholar.umt.edu.pk/handle/123456789/3177
dc.language.isoenen_US
dc.publisherapplied surface science, Elsevieren_US
dc.subjectFood and Agricultural Scienceen_US
dc.subjectSilver nanoparticles, Anti-biofilm agents, Heliotropium crispum, Synthesis, Bactericidal effect, Green synthesisen_US
dc.titleControlled assembly of silver nano-fluid in heliotropium crispum extract: a potent anti-biofilm and bactericidal formulation.en_US
dc.typeArticleen_US
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