Gas chromatogrphy flame ionization detector physico chemical studies of oil from the roots of moringa oleifera
No Thumbnail Available
Date
2019
Authors
RIZWAN HABIB
Journal Title
Journal ISSN
Volume Title
Publisher
UMT, Lhr
Abstract
Moringa oleifera is an important medicinal and a multi-nutritional plant being useful for the treatment of a variety of diseases. This research evaluates the physico chemical properties of Moringa oleifera root oil by standard procedure. The fatty acid profile was measure by gas chromatography flame ionization detector analysis, also study was carry out at the antioxidant activity and antimicrobial activity of oil from the roots of Moringa oleifera. The Moringa oleifera root oil was extracting through reflux by using n-hexane. The liquid black colored oil was obtaining with % yield of 5.1906. Saponification number and acid value are 208 and 7.0775 respectively. The chromatogram of GC-FID analysis indicated the presence of various fatty acid. The main fatty acids were palmitolic acid (28.4442 %), linoleic acid (52.9744 %), linolenic acid (3.2052 %), archadic acid (3.3438 %), behenic acid (2.962 %), and cerotic acid (1.677 %). Antioxidant and antibacterial activity were carry out using 2, 2-diphenyl-1 picryhtdrazyl (DPPH) assay and well diffusion method respectively. The maximum concentration of 50 uL showed maximum % inhibition about 87.0421%. Antimicrobial activity of plant root oil was testing by disc diffusion method against bacterial strain such as Staphylococcus aureus, Bacillus subtilis and E. coli. It exhibited strongest activity against E. coli and Staphylococcus aureus. While no activity observed against Bacillus subtilis. The oil of Moringa oleifera contains low and high molecular weight, fatty acids, which are suitable for the application in soap, cosmetic industries and pharmaceutical industries.