Synthesis and characterization of sanguinarine nitrate

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dc.contributor.authorHassan Askary, Syed
dc.date.accessioned2018-08-06T05:03:11Z
dc.date.available2018-08-06T05:03:11Z
dc.date.issued2017
dc.descriptionSupervised by: Dr. Sohail Nadeemen_US
dc.description.abstractSanguinaria canadensis, common name bloodroot have eight isoquinoline alkaloids with six main alkaloids names as quaternary ammonium benzophenanthridine are sanguinarine, chelerythrine, sanguilutin, chelilutin, sanguherin, and quetririn in them sanguinarine alkaloid concentration is highest that is approximatly 50 %. Sanguinarin alkaloid was separated by precipitation method and sanguinarine nitrate was synthesized by repeated acid base reaction. In which concentrated amonia and dilute nitric acid was used. Conformation test was performed on HPLC and UV-vis. A shortest time period method was introduced on HPLC. Sanguinarine was separated on Zorbax Eclipse Plus C18, 4.6 x 100mm, 3.5 micron column, by using gradient elution of ACN (A) in 1% acetic acid in water (B). For quantification, Chromatograms were acquired at 254nm. 10µg/ml injection was passed repeatedly three times. Results showed peak area at 1.43±0.02 retention time. Nitrate confirmation was confirmed by salt analysis while in UV-vis spectrophotometer standard and sample was run at 300 nm from which concentration of nitrate was also determined.en_US
dc.identifier.urihttps://escholar.umt.edu.pk/handle/123456789/3101
dc.language.isoenen_US
dc.publisherUniversity of Management and Technologyen_US
dc.subjectchelerythrine, sanguilutin, chelilutinen_US
dc.subjectQuaternary ammonium benzophenanthridineen_US
dc.subjectMSen_US
dc.titleSynthesis and characterization of sanguinarine nitrateen_US
dc.typeThesisen_US
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