Synthesis, characterization and biological Applications of metal complexes of omeprazol
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Date
2016
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UMT, Lhr
Abstract
Background: Omeprazole is a class of substituted benzimidazole and proton pump inhibitor used in the treatment of various diseases like dyspepsia, peptic ulcer disease, gastroesophageal reflux disease and Zollinger Ellison syndrome for medical healing actions and prevention of relapse. It is an excellent example of prodrug with a specific drug and possesses the high degree bio-activation. In the previous studies formation of metal complexes with omeprazole ring containing ligands because omeprazole and its derivatives play very important role in several biological reactions. The importance of drugs and their complexes have been desired to synthesize and characterize some ternary complexes of inner transition metals with an omeprazole and its derivative. Methods: Metal complexes of omeprazole (OMZ) have been prepared by the synthetic reaction of the OMZ with the Zn(II), Cu(II) and Ni(II) salts of sulphate within the metal to OMZ molar ratio is 1:2 and attached the co-ligand (2,2´-bipyridine) to the Metal-OMZ complexes with the molar ratio of 1:1 (co-ligand to metal-OMZ). These complexes gave the sharp melting point indicating the isolation of fairly pure complexes.Results: The molar conductance data of the OMZ and synthesized complexes revealed that the Metal-OMZ chelates are non-electrolytes but Metal-OMZ-bipy chelates are electrolytes in nature. The complexes were characterized by FTIR, UV-visible, and conductance measurement. FTIR spectroscopy showed that OMZ acts as a neutral bidentate ligand through the pyridinic nitrogen of the benzimidazole ring and the oxygen atom of the sulfoxide group, forming a five-membered ring chelate. Electronic and FTIR spectra indicate a distorted octahedral geometry around Zn-OMZ and Cu-OMZ, where the coordination sphere is completed by two water molecules and Ni-OMZ complex have tetrahedral geometry around the metal ions. But all Metal-OMZ-bipy complexes have the octahedral geometry due to attachment of 2,2՛-bipyridine. Conclusion: The effects of these complexes were investigated by alkaline phosphatase (ALP) activity on the blood serum. The alkaline phosphatase activities in a control serum sample concurrently treated in the same manner and bind the active site of the enzyme. It was also found that at a concentration of 7.317 μM the ALP inhibition was 80-90 % for all complexes. The DPPH activity of Metal-OMZ-bipy complexes showed the higher scavenging activity then the OMZ (L), Zn(II), Cu(II) and Ni(II) OMZ complexes. These indicated that the Metal-OMZ-bipy complexes have much stronger radical scavenger than the OMZ, metal-OMZ complexes as well as ascorbic acid (IC50 = 40.31±0.4166 μM). In vitro studies of OMZ and their synthesized complexes were tested against the bacterial species as Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens, Klebsiella pneumoniae and fungal specie against Candida albicans. Metal complexes were found to show better activities as compared to the ligand and the standard drug used as control.