Biodegradation of phenol by stenotrophomonas sp. And staphylococcus sp. Isolated from contaminated sites.
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Date
2016
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Applied Ecology and Enviromental Research
Abstract
Phenol as environmental pollutant is detrimental to living organisms and needed to be
eliminated for environmental safety. Among the various practiced approaches for its removal, bacterial
utilization gets attraction due to its eco-friendly and cost effective nature. For this purpose, bacterial
strains were isolated from bioremediation site and industrial waste through enrichment in phenol (250 mg
L
-1
) for 3 days at 28oC. After enrichment, morphologically distinct colonies were purified on phenol (200
mg L-1
) agar plates and the strains were identified through 16S rRNA gene sequence. Total of eight
strains were identified, among them two strains, NCCP-310 and NCCP-405 had the best potential of
phenol degradation which were identified as the members of the genera Stenotrophomonas and
Staphylococcus. NCCP-310 and NCCP- 405 showed 98.85 and 98.9% sequence identity with
Stenotrophomonas maltophilia and Staphylococcus equorum subsp. equorum, respectively. Both strains
have ability to tolerate 1000 mg L-1
phenol. The isolated strains degraded 750 mg L-1
of phenol at pH 7
and 28+2oC. NCCP-310 and NCCP-405 showed degradation of such amount in 65 and 85 h with the
average rate of 15.65 and 11.64 mg L-1
h
-1
. Our work suggests that these strains are efficient in phenol
removal and could be used for bioremediation.
Description
Keywords
Food and Agricultural Science, phenol, biodegradation, Stenotrophomonas, Staphylococcus, bioremediation.
Citation
Ahmad, N., Ahmed, L., Iqbal, M., Khalid N., Abbas, S., Mehboob, F., & Ahad, K. (2016). Biodegradation of phenol by Stenotrophomonas sp. and Staphylococcus sp. isolated from contaminated sites. Applied Ecology and Enviromental Research, 14(5), 107-120. (Nauman Khalid (SFAS), JCR LISTED (IF: 0.68))