Extraction of oleic acid from natural sources
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Date
2016
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UMT, Lhr
Abstract
Fatty acids are important for biotechnology industries from many years. These fatty acids were obtained from different sources such as plants and animal fats. Production of fatty acids was done by different chemical methods that were costly and time consuming. In this study potassium hydroxide catalyzed reactions were utilized to hydrolyze mustard oil. Different parameters for percentage of free fatty acids were investigated (temperature and reaction time) and optimum conditions for maximum free fatty acids percentage was achieved.
This hydrolyzed mixture of free fatty acids consisted of erucic acid, oleic acid, omega-3 alpha-linolenic acid and omega-6 linoleic acid. Mixture of free fatty acids was treated with methanol and urea and then filtrated to get high purity oleic acid. After separation of high purity oleic acid chemical tests (saponification value, acid value and ester value) were performed and results shows that these values are 168.3, 157.08 and 11.22 respectively. GC-MS shows only one major peak of oleic acid having retention time 7.58 mints.