Free radical scavenging activity of verbena bonariensis
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Date
2017
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University of Management and Technology Lahore
Abstract
Verbena bonariensis (purpletop vervain) is a plant belongs to verbenaceae family. It is approximately 18 to 36 inch tall plant along with rigid branching stem having height 2 to 3 inch, purple colour flowers also observed on this plant in spring, summer and autumn season. It is used as a natural preservative rather than synthetic one. To ensure effectiveness of this plant the antioxidant action of five different fractions i.e. “chloroform, ethyl acetate,n-hexane, n-butanol and aqueous samples” of said plant were prepared and evaluated by using five different methods i.e “DPPH radical scavenging activity, total antioxidant study, ferric reducing antioxidant power analysis (FRAP), total phenolic contents and ferric thiocynate (FTC) assay”. Results shown that ethyl acetate soluble portion articulated maximum percentage inhibition of DPPH radical i.e 80.9 ± 0.87% inhibition at a concentration of 30μg/mL as compared to other fractions. While FRAP analysis result indicated the highest antioxidant activity along with n-butanol fraction i.e 322 ± 1.39 TE/μM , the other three methods effect indicated the highest antioxidant value against the ethyl acetate fractions. Enzyme inhibition activity of five fractions were evaluated by using enzymes like α- glucosidase, butyralcholinesterase, acetylcholinesterase and lipoxygenase, results compared with quercetin having IC50 value 16.47±0.39, eserine having IC50 value 0.86±0.002, eserine having IC50 value 0.04±0.001 and baicalien having IC50 value 22.69±1.41 respectively. n- butanol indicated good activity (68.68±0.55 % inhibition with conc. 0.1 mg/mL) against α- glucosidase. Ethyl acetate indicated fine activity against butyralcholinesterase and lipoxygenase (71.45±0.65 & 66.75±0.91 with conc. 0.1 mg/mL) respectively. Aqueous fraction illustrated good activity against acetylcholinesterase (37.61±0.53 with conc. 0.1 mg/mL). Hence, this research confirmed that V. bonariensis is a valuable plant, which is used in many natural preservatives also beneficial in preparation of many folk medicines due to its significant antioxidant results.
Description
Supervised by: Dr. Sammia Shahid
Keywords
Verbena bonariensis, Verbenaceae family, DPPH radical scavenging, MS Thesis