Clarithromycin nanoparticles synthesis, characterization and enhancement of antimicrobial activities and dissolution rate
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Date
2017
Authors
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Publisher
University of Management and Technolog
Abstract
Low solubility and bioavailability was the major concern in the past for macrolide antibiotic drugs. The solubility and dissolution rate could be enhanced by preparing nanodrugs as their surface area increases. In this research clarithromycin drug nanoparticles were prepared by using solvent-antisolvent precipitation method. The prepared drug nanoparticles were characterized by FTIR, SEM, EDX and XRD. Dissolution rate was studied by using Dissolution apparatus and High Performance Liquid Chromatography (HPLC) at different time intervals was used. The dissolution rate of nano drug showed a major enhancement as compared with the parental drug. No difference was observed in IR spectra of both parental drugs and nano drug of clarithromycin. X-Ray diffraction (XRD) showed the amorphous form of clarithromycin. The SEM images showed the nanosized particles of nanodrug. The antibacterial activities of both parental drug and nanoparticles were determined against Staphylococcus aureus, E. coli, and Salmonella typhi by agar well diffusion method. The nano drug showed better results as compared to the parental drug.
Description
Supervised by:Dr. Sammia Shahid
Keywords
Clarithromycin; High Performance Liquid Chromatography, Nanodrugs; Macrolide Antibiotics, MS thesis