Screening of camellia sinensis (black tea) for its active constituents
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Date
2016
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UMT, Lhr
Abstract
Camellia sinensis (C. sinensis) L. is a tea plant which is most drunk beverage in the world. India, china and Iran are famous for the cultivation of tea. C. sinensis is a medicinal plant which belongs to the theaceae family. Its medicinal value was proved by alkaloids, flavonoids, saponins and tannins present in its leaves extract. These all compounds of this plant are very effective and have pharmacological effect such as anticancer, analgesic and antiviral. In Pakistan these infectious diseases are so common because bacterial strains have been developing with the passage of time and they show resistance to all antibodies. So we should make more antimicrobial agents that have minimum effect. Camellia sinensis extracts showed both antibacterial and antioxidants activities. From the last two decades most of the work has been done on these two activities. As it is found in Pakistan on wide land so it should be used as medicine in an economical way.
Three brands of black tea (Tapal, Supreme and Lipton) were taken and their extracts were prepared in methanol, ethanol and in aqueous. Aqueous extracts were prepared at three temperatures 70oC, 80oC and 100oC. All the extracts showed significant activity against three bacterial strains. Samples of Tapal showed good results in all solvents against E. coli in the range of 7mm to 20mm. Low activities were displayed by Lipton in the range of 7mm to 12mm against E. coli. Whereas Supreme behaved consistently in aqueous extract at all temperature but in compared to ethanolic and methanolic solvents it gave higher activity against E. coli. The highest activity was shown by alcoholic samples of Tapal and Supreme. Values of Tapal ranges from 13mm to 20mm and for Tapal it was from 17mm to 20mm that indicate that alcoholic extracts are good source of medicine against viral disease. Antioxidant activity was performed of these extracts using gallic acid as standard. Absorbance showed that which sample is more antioxidant. Maximum absorbance was shown by Tapal black tea in methanol which was 2.5885.