Epitope Mapping of HCV Structural Proteins for Potential Vaccine Design; an Insilico Approach
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Date
2018
Authors
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Publisher
University of Management & Technology
Abstract
Hepatitis C virus (HCV) is a leading cause of morbidity and mortality globally. Recent studies have shown an increased burden (2.8%) of HCV infection, which is nearly equal to 185 million infections globally. Highest prevalence rated countries are located in Asia and Africa. Pakistan has the high HCV seroprevalence rates between 2.4% and 6.5% in general population. Due to error prone nature, HCV has the ability to escape immune system surveillance. High variation in genome is the primary hurdle in vaccine development against the virus. After the infection host, Cytotoxic T- lymphocytes contribute their role in the eradication of infection. For successful eradication of HCV a vaccine development is a need of the hour. HCV Structural proteins are one of the potential immunogenic viral proteins. The study is aimed to analyze the core, E1 and E2 protein as a potential vaccine candidate. In this study the sequences of HCV structural proteins were taken and comparison was made by BLASTP to find out maximum potential candidates for a vaccine development. All the sequences were aligned by using CLUSTAL OMEGA software and consensus sequence was developed by using JALVIEW software. Immune Epitope Database (IEDB) was used to predict epitope sequences. The predicted epitopes were analyzed to determine their antigenicity through Vaxijen server version 2.0. I- Tasser software further used for the visualization of predicted epitopes positions and immunogenicity.
Description
Dr. Muhammad Sohail Afzal
Keywords
HCV; Core protein; Immunity; Epitope; Vaccine; Insilico, MS