INCIDENCE AND SUSCEPTIBILITY PATTERN OF KLEBSIELLA SPECIE FROM DIFFERENT CLINICAL SAMPLES
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Date
2016
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UMT, Lhr
Abstract
K. pneumonia is one of the specie of Enterobacteriaceae family which is the cause of most of the community acquired and nosocomial infection. The prevalence of Klebsiella infection is because to its resistance towards different antibiotics. Klebsiella contains beta lactamases enzyme which shows resistance to beta lactam antibiotics. There for its prevalence and incidence is different in different region of the world. Klebsiella is considered as second in raking, among gram negative bacteria which cause nosocomial infection. It is broadly distributed among hospital patients and 3 to 8 % nosocomial infections are due to Klebsiella.The prevalence of Klebsiella is associated with the other kind of infection such as respiratory tract infection, urinary tract infection and soft tissue infection.Antibiotic Susceptibility has been determined againstinfection produced by Klebsiella species at various infection sites.
Method: The samples were obtained from the various sites of infections such as from Blood, URTI, UTI and wound infection from the Jinnah hospital Lahore. The samples of Klebsiella species infections have been tested for the confirmation of Klebsiella specie presence by using the various kinds of biochemical test such as Gram staining, Lactose fermentation test, Motility test, Catalase test and Colony formation on MacConkey agar, indole test, urease test, Vogas proskauer test, Citrate utilization test. The susceptibility pattern and resistance pattern has also been evaluated by using eighteen different antibiotics. Mullar Higton agar is used for the determination of Susceptibility and Resistance pattern. The susceptibility and resistance pattern has also been determined for each sites of infection. The effect of one antibiotic has been tested on varies sites of infections and results are compiled to determine which antibiotic is most effective against each site of infection.
Result: The forty antibiotic are used for Susceptibility while eighteen antibiotics selected on the basis of the number of sample tested and their results are compared against each site of infections. The result showed that the effect of one antibiotic against various infection site is not same its susceptibility varies with the site of infection. The most susceptible antibiotic is clindamycin 100% while the most resistant antibiotic is Ofloxacin 100%. The results with respect of site of infection showed that the most susceptible antibiotic for blood infection is Ciprofloxacin 28.6% for upper respiratory tract is Amoxicillin and Clindamycin which are 100% and 90.5 % susceptible respectively. For urinary tract infection the most susceptible is the Cotrimoxazole which sensitivity is 43%. For wound infection the most susceptible drug is Gentamicin minocycline and ceftriaxone which are respectively 75%, 67% and 54% susceptible.
Conclusion: It is concluded from the above study that the effect of each antibiotic is different with respect of site of infection and kind of infection so it is recommended that the doctor should determine the susceptibility test before to prescribe the antibiotic which would help us to cure from the disease in few days. This study confirms that the susceptibility pattern varies from person to person and with different sites of infection of the same person. This study should be conducted on the large scale to determine the susceptibility of antibiotics against each kind of infection and should be implemented on the recommendations of the study at national level.