In silico characterization of hypothetical proteins of treponema pallidium
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Date
2022
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UMT, Lhr
Abstract
Treponema pallidium is a gram-negative bacteria with corkscrew-shaped and its optimal narrow ranges of pH is 7.2 to 7.4. It expands in moist regions of the body. It is a small, motile organism that is not visualized by light microscopy. German zoologist Fritz Schaudinn and dermatologist Erich Hoffman observed this bacteria in diseased tissues. It was first recorded in Europe. Globally, 7 million new cases of syphilis were estimated in 2020. Till, 2030 to reduce the incidence of syphilis by 90%, WHO has set targets but the global response has been slow. In this study, 44 hypothetical proteins of T. pallidium have been targeted for functional and structural annotation. To check the template accessibility the selected proteins were first subjected to homology screening. After filtering 25 hypothetical proteins were evaluated further for conserved domain analysis through CDD, Pfam, InterProscan, and Scanprosite, physiochemical characterization via ExPASy ProtParam, subcellular localization via CELLO v2.5, and PSORTb v 3.0, and structural modeling via I-TESSER and SAVES server (v.5.0). The pathogenicity assessment using VirulentPred and functional annotation through CATH v4.3 and Argot v2.5 shown that 21 out of 25 hypothetical proteins could act as potential virulent factors. The finding of this study could assist in confirming the functions and pathogenic involvement of these proteins and may help target in identification for drug discovery and vaccine designing for T. pallidium in near future.