Phytochemical and pharmacological evaluation of cotula anthemoides l.
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Date
2019
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UMT, Lhr
Abstract
Cotula anthemoide is a famous plant for medicinal purposes. Presence of bioactive molecules makes it active against pathogens. The aim of present work was to evaluate antimicrobial and antioxidant activity of Cotula anthemoides extract fractions against bacterial strains. Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Bordetella bronchiseptica microbial strains were used for antimicrobial activity. Leflox was used as positive control. All fractions n-hexane, chloroform, ethyl acetate and n-butanol were active against pathogenic organisms. To check the effectiveness of Cotula anthemoide antioxidant activity were performed for all fractions by DDPH (free radical scavenging activity) and FRAP (ferric reducing power analysis). Results showed that ethyl acetate and chloroform soluble fractions have highest percentage of DDPH free redical as compared to n-hexane and n-butanol fractions. While results of FRAP activity showed that only ethyl acetate (298.3 TE µM/ml) soluble fraction gives highest inhibition as compared to n-hexane, chloroform and n-butanol soluble fractions. For the extraction of essential oils hydro distillation method was used. Time of extraction of oil was 4 to 5 hours. GC-MS technique was used for the evaluation of oils. Results of GC-MS showed the presence of the aromandendrene (6.646 min), caryophyllene (5.933 min), β-bisabolene (8.771 min) and caryophylene oxide (7.834 min) retention time. Aromandendrene, caryophyllene have greater concentration in oils of leaves and stems.