Evaluation of tomato hybrids for the antioxidant potential and parental confirmation using molecular markers
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Date
2022
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UMT, Lhr
Abstract
The tomato (Solanum lycopersicum L.) is a fresh and processed fruit in the world. Tomato can be ranged as the 2nd most cultivated and consumed fruit round the globe. These foods are rich in dietary fiber, carbs, amino acids, minerals, and vitamins. Iron, phosphorus, vitamin B, and vitamin C are all abundant in tomatoes. Regular consumption of tomato can decrease the risk of different chronic and heart related diseases because of antioxidants like lycopene, carotenoids and vitamin C. Molecular confirmations of the hybrids were carried out in study to confirm true hybrids. DNA was extracted from the young seedlings using CTAB method. Different DNA based markers systems like RAPD, SSRs, URP and SCAR were used to detect polymorphism in the F1 hybrids. To collect the data software was used that was statistic8.1. In which the different five varieties were used these were NBH 228, NBH 151, NBH 152, NBH 196, NBH 204 for all these varieties total quantities of the total phenolic content, reducing sugar, non-reducing sugar, chlorophyll content, carotenoids content and total sugar was checked that which variety have the much more significant while comparing them with each other. PCR was performed to get different bands which shows that either our varieties are true to hybrid or not. In the findings the five different bands were obtained for all the varieties. If these are true hybrids, then they must have the male bands if male band is not present that’s mean it is self-pollinated but not the true hybrid. If male band is shown in all varieties that show that all varieties are true to hybrid. This result shows that all these varieties are true to hybrid. Biochemistry was performed is correlate with this gel that
show these are true hybrids that’s why they are different from their parents