Department of Chemistry

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Analysis of gyra mutations in local TB patients of Lahore Pakistan
(2017) Iram Afzal
Tuberculosis is a slowly progressive, debilitating disease that kills about 1.5 million people world-wide. Pakistan ranked 4th in global TB burden. In this research work, male-female ratio showed that the rate of TB is 32 times higher in women as compared to men. The patients suffering from pulmonary TB were 94% and only 6% were recorded as extra pulmonary TB. All the extra pulmonary TB cases were found in females. There were about 72% cases which were positive for MDR-TB. Out of these72% MDR-TB cases,all were previously treated. The differentiation between M. bovis and M. tuberculosis reflects that none of the sample belonged to M. bovis. All the isolates were belonged to M. tuberculosis, but 6% samples had extra-pulmonary TB. It shows that M. tuberculosis not only affects the lungs, but also the other parts of the human body. GyrA mutations were investigated by using newly designed primers, at codons 90, 91, and 94. Results showed that out of all samples there were mutations in 60% samples. There were mutations in 30% samples at codon 90, 6% samples were mutated at codon 91 and 24% were mutated at codon 94.