School of Science (SSC)

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Browsing School of Science (SSC) by Subject "ALP assay"

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    Synthesis, spectroscopic studies and biological applications of zn(ii), cd(ii) & co(ii) carboxylates
    (University of Management and Technology Lahore, 2017) Farhan Butt
    Background:Metal carboxylates belong to that kind of chemical substances comprising of metal and the long-chain unsaturated and saturated fatty acids. The catalysis of the metal is connected and utilized as a curing promoter and attachment promoter.These metal carboxylates act as accelerator for the chemical reaction and used as inhibitors in the treatment of various diseases like controlling apoptosis, metabolism of genome and proteins, stabilizing macromolecules, controlling bacterial gene expression by blocking the synthesis of proteins. It is an excellent example of prodrug with a specific drug and possesses the high degree bio-activation. At this time, the use of metal carboxylates is greatly popular in the paints, drying and detergents etc. They are especially used in the formation of materials having waterproofing qualities. Hardness of organic substances can also changed by them. Regardless of having long history, the interest for the science of metals and their carboxylates has endured and there is a need of investigating their basic caracteristics, for example, carboxylate bonding modes, crystal structures, thermal properties and biological applications.Metal carboxylatesplay very important role in various biological reactions.Significance of drugs and their complexes have been desired tosynthesize and characterize some ternary inner transition metals complexes with carboxylate and their derivative, Methods: Solid complexes of carboxylates were prepared by the reaction of metal salts containing Zn(II), Cd(II), Co(II) and ligand in the ratio of 1:2. Then ethylenediammine (co-ligand) attached to the Metal-carboxylate complexes with the molar ratio of 2:1 (co-ligand to metal-carboxylate). Results:Pure complexes have been isolatedhaving sharp and fixed melting point. The complexes were characterized by FT-IR and conductance measurement. FT-IR spectroscopy supported the non involvement of amino nitrogen of the ligand HL with metals because vibrational frequency of NH was not shifted to a considerable extent in complexes 3–8 as compared to the free ligand (HL).The molar conductance data of the synthesized complexes revealed that the ligand is non-electrolyte but Zn(II), Cd(II) & Co(II) Carboxylatesare electrolytic in nature. A distorted octahedral geometry was observed around Zn and Co- complexes, where, four oxygen atoms and two water molecules complete the coordination sphere. All Metal-carboxylates coligand complexes have the octahedral geometry due to attachment of ethylene diammine. Conclusion:The effects of these complexes were applied on alkaline phosphatase (ALP) and their activity on the blood serum was also checked.The alkaline phosphatase activities in a control serum sample and active site of the enzyme bind by it.It was also noted that at a concentration of 4mM the inhibition of ALP was 90 % for all the complexes.It has been noted that most of the metal complexes are more active than their respective ligand showing that activity of the ligand enhanced by thecomplexation.