Browsing by Author "NOOR UL AIN"

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    Inclusive education and curriculum adaptation
    (UMT, Lahore, 2023) MUHAMMAD MUEEN NAWAZ; NOOR UL AIN
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    Isolation and characterization of amylase producing bacteria and optimization of their amylolytic activity
    (UMT, Lhr, 2022) NOOR UL AIN
    The present work deals with the amylase enzyme producing bacterial stains. For the isolation of stains producing amylase, samples were collected from vegetable markets of different areas. The isolated colonies were screened by clear zone formation on starch agar using iodine solution. Out of 10 bacterial stains, 2 showed the best results. The bacteria were characterized morphologically and biochemically. Bacteria growth was optimized, and it was found that the best growth was found at pH 7 and temperature 37°C. Amylase enzyme activity by these bacteria was quantified using o-toluidine method. The optimal conditions for amylase activity were also determined and it was found that the bacterial isolates V4 and V5 showed maximum at amylase activity at pH 7 and temperature 37°C. Enzyme activity was also determined in the presence of different metals, and it was found that best results were shown in the presence of Ca as compared with others. Genomic DNA was isolated and 16S rRNA gene PCR was performed followed by its sequencing. The sequence analysis revealed the identity of the bacterial isolate V5 as Rothea amarae.
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    Isolation and characterization of oil degrading bacteria from oil contaminated sites
    (UMT Lahore, 2023-08-31) ZOHAA YASIN; SARA AFZAL; NOOR UL AIN
    Crude oil spills wreak havoc on both land and water, poisoning soil, rivers and oceans. So, in this case Bioremediation is a promising option which uses nature's ability to break down pollutants. Oil-eating bacteria and other specialized microorganisms are used to break down oil molecules into harmless byproducts. This natural decomposition process reduces the long-term environmental damage caused by oil pollution. A total of 7 bacterial isolates were obtained from mechanic shop soil in this study. These isolates were characterized and further studied to evaluate their ability to break down crude oil and enhance plant growth. Bacteria were cultivated in minimal salt media (MSM), with crude oil as the main carbon source. Morphological and biochemical analysis was performed on the isolated bacteria. Bacterial growth was monitored by measuring optical density, which increased over time. The results of this investigation emphasize the valuable potential of oil-degrading bacteria in bioremediation techniques and sustainable agricultural practices. Furthermore, the presence of these oil-degrading bacteria did not adversely affect the growth of plants but supported it. Thus, we can say this study highlights the synergy between ecological restoration and plant growth, offering a holistic approach to addressing both environmental and agricultural challenges. By harnessing the natural strengths of these bacteria, it is possible to reduce hydrocarbon pollution as well as restore agricultural productivity and soil health.
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    Removal of cyanide by solid phase extraction from aqueous medium using biochar based adsorbent
    (UMT, Lhr, 2022) NOOR UL AIN
    Water pollution is a major problem that affects millions of people worldwide and poses a threat to entire ecosystem. Water pollution not only depleted natural resources but is also one of the major worldwide risk factors for sickness, disease and mortality. A large quantity of cyanide is present in water due to human activities such as metal finishing and mining industries. Biochar has untapped technology for drinking water because it has excellent capacity for the removal of containments. This present study is based on biochar derived from banana peel for the removal of this toxic anion. Biochar is prepared by pyrolysis at 400 to 500 OC and is activated by ZnCl2 and SDS. Ion selective electrode used to study the batch wise adsorption which is done by different parameters such as kinetics, thermodynamics, Langmuir isotherm, adsorbent dose, time of contact, and pH. Characterization was done by Scanning electron microscope (SEM), FTIR, TGA, CHNS showed the adsorption capacity of biochar, The experiment show that biochar derived from banana peel and grapefruit peels shows efficient adsorption for cyanide and they are eco-friendly and cost effective too.