Isolation and characterization of amylase producing bacteria and optimization of their amylolytic activity
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Date
2022
Authors
NOOR UL AIN
Journal Title
Journal ISSN
Volume Title
Publisher
UMT, Lhr
Abstract
The present work deals with the amylase enzyme producing bacterial stains. For the isolation of stains producing amylase, samples were collected from vegetable markets of different areas. The isolated colonies were screened by clear zone formation on starch agar using iodine solution. Out of 10 bacterial stains, 2 showed the best results. The bacteria were characterized morphologically and biochemically. Bacteria growth was optimized, and it was found that the best growth was found at pH 7 and temperature 37°C. Amylase enzyme activity by these bacteria was quantified using o-toluidine method. The optimal conditions for amylase activity were also determined and it was found that the bacterial isolates V4 and V5 showed maximum at amylase activity at pH 7 and temperature 37°C. Enzyme activity was also determined in the presence of different metals, and it was found that best results were shown in the presence of Ca as compared with others. Genomic DNA was isolated and 16S rRNA gene PCR was performed followed by its sequencing. The sequence analysis revealed the identity of the bacterial isolate V5 as Rothea amarae.