Browsing by Author "Ayesha Sadiqa"

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    Classification of yoga postures using mobilenet and efficientnetb0
    (UMT Lahore, 2024) Ayesha Sadiqa
    Over the past few decades, yoga classification has become an important problem for many reasons. The growing popularity of yoga has created a demand for tools that accurately assess yoga poses to ensure correct performance, prevent injuries, and enhance benefits. Limited access to expert instructors highlights the need for affordable solutions offering real-time feedback. Existing products like smart mats and wearables may be uncomfortable or costly. Thus, a machine learning-based system is essential to make quality yoga instruction and assessment accessible to all. In this study, we propose a novel approach for yoga pose classification using a combination of two deep architectures, namely: MobileNet and EfficientNetB0. While our results demonstrate that the proposed model outperforms other compared models, achieving the highest accuracy of 97.65% on the Yoga82 dataset, the approach may face challenges such as computational requirements for real-time implementation and reduced performance in cases of poor-quality input images. These limitations highlight areas for further improvement in future research.
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    Cloning, expression and characterization of α-amylase gene from pyrobaculum calidifontis
    (UMT, Lhr, 2017) Ayesha Sadiqa
    The present study describes the cloning, expression and characterization of alpha amylase from Pyrobaculum calidifontis. α-amylase is an extracellular enzyme and belongs to GH13 family. It is a starch degrading enzyme which hydrolyzes the α-1, 4-glycosidic linkages. In recent biotechnology applications the enzyme is being used in fermentation, food, paper and textile industry. Cloning of alpha amylase (Pcal_1039) gene was done in expression vector pET-21a(+). Pcal_1039 gene was expressed in E. coli BL21 and analysis of protein was done on SDS-PAGE. Recombinant protein was refolded by using in vivo and in vitro methods. The size of the gene was 1.37 kb and molecular mass of protein was 53.5 kDa. After expression the activity of α-amylase was measured by DNS method at OD540. Before refolding the inclusion bodies showed a minor activity while highest activity of 3.3 U/ml and specific activity of 23.6 U/mg/ml was measured by taking expression at 17°C (in vivo).
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    Cloning, expression and characterization of α-amylase gene from pyrobaculum calidifontis
    (University of Management and Technolog, 2017) Ayesha Sadiqa
    The present study describes the cloning, expression and characterization of alpha amylase from Pyrobaculum calidifontis. α-amylase is an extracellular enzyme and belongs to GH13 family. It is a starch degrading enzyme which hydrolyzes the α-1, 4-glycosidic linkages. In recent biotechnology applications the enzyme is being used in fermentation, food, paper and textile industry. Cloning of alpha amylase (Pcal_1039) gene was done in expression vector pET-21a(+). Pcal_1039 gene was expressed in E. coli BL21 and analysis of protein was done on SDS-PAGE. Recombinant protein was refolded by using in vivoand in vitro methods. The size of the gene was 1.37 kb and molecular mass of protein was 53.5 kDa. After expression the activity of α-amylase was measured by DNS method at OD540. Before refolding the inclusion bodies showed a minor activity while highest activity of 3.3 U/ml and specific activity of 23.6 U/mg/ml was measured by taking expression at 17°C (in vivo).