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Browsing SOP by Author "Hooria Gul"

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    ANTIEPILEPTIC AND NEUROPROTECTIVE POTENTIAL OF ACACIA NILOTICA (KEKKAR)
    (UMT, Lahore, 2025) Hooria Gul
    Epilepsy remains a major global health challenge, with approximately one third of patients experiencing drug resistant seizures and enduring adverse effects from conventional antiepileptic drugs. This study evaluated the antiepileptic and neuroprotective potential of an ethanolic bark extract of Acacia nilotica (AnEth) in Balb/c mice subjected to Pentylenetetrazol (PTZ) induced kindling. Phytochemical profiling by FTIR, LC-MS/MS, and GC-MS confirmed a rich composition of polyphenols, flavonoids, tannins, and triterpenoid saponins. The extract exhibited strong antioxidant activity (88%DPPH scavenging at 10 mg/mL) and was found to be safe up to 2000 mg/kg in an acute oral toxicity assay. In the chronic PTZ model(40 mg/kg,i.p.,on alternate days for 21 days),mice were randomized into five groups(n=8).Seizure incidence,latency,duration,and modified Racine scores were recorded, alongside behavioral assessments)and body weight monitoring alone induced seizures in 87.5% of mice, with mean Racine scores rising to 3.3±1.3.Diazepam fully prevented seizures, whereas AnEth at 200 mg/kg and 400 mg/kg reduced seizure incidence to 50% and 37.5%,and mean Racine scores to 1.25±0.5 and 1.7±1.1,respectively (p<0.01 vs. PTZ). Both AnEth doses ameliorated PTZ induced weight loss and improved anxiety like behaviors. The extract’s favorable safety profile and multifaceted neuroprotective actions support its potential development as alternative therapy for refractory epilepsy. Plant extract shows promising antioxidant trends, PTZ treatment caused oxidative stress in the brain, shown by a rise in antioxidant enzymes like Catalase and SOD