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Isolation and growth optimization of heavy metal resistant bacteria for bioremediation of mercury
(UMT Lahore, 2024-10-02) RAHIMA GUL; AGHA HASSAM ALI KHAN
Heavy metals must be removed from wastewater using environmentally friendly techniques; however, these techniques are expensive. Due to human activity, chemical pollutants that are extremely dangerous to living forms have leaked into the environment. This study provides information on different heavy metal-resistant bacteria for the bioremediation of different metallic pollutants. An overview of the integrated approach is given here, which treats many heavy metals and other environmental toxins at the same time by using different heavy metal-resistant microorganisms. In this study seven isolates RH1, RH2, RH3, RH4, RH5, RH7 & RH8 were isolated. After series of biochemical test, the strains RH1, RH2 & RH5 were observed as they have the potential to resist against heavy metal. These strains show positive results again catalase test but observed as negative in case of mannitol fermentation, starch hydrolysis, TSI & simon citrate agar test. These isolates showed MIC up to 15mM concentration and reveal positive results in case of temperature, ph. The mercury reduction assay of the selected isolates showed the Hg utilization in form of changing of color from green to orange.