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Browsing BS by Author "ADAN REHMAN"

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    Isolation, identification & characterization of bacterial strain from industrial effluents
    (UMT Lahore, 2024-10-04) ESHA TARIQ; M.ARSLAN NAWAZ; MAIRA KHAN; ADAN REHMAN
    The contamination of water bodies by industrial effluents poses significant environmental and public health challenges, particularly in developing regions. To solve these problems, bacterial strains were isolated from effluents collected from the drainage pipes of Chaman Industries, Lahore, Pakistan. To selectively isolate extremophiles, serial streaking was performed on alkaline media at pH 9. Morphological identification of the bacterial strain was conducted through various staining techniques and molecular identification through 16S rRNA sequencing confirmed the strain as Exiguobacterium profundum. A comprehensive set of biochemical tests were performed to further characterized the bacterium's metabolic capabilities. Bioinformatic analysis revealed the upregulation of the gene mtlD, encoding the enzyme Mannitol-1-phosphate 5-dehydrogenase, which catalyzes the conversion of D-mannitol 1-phosphate to D-fructose 6-phosphate. This enzyme, essential for mannitol biosynthesis, likely plays a key role in the bacterium’s adaptation to extreme environmental conditions. Additionally, the bacteria showed significant resistance to heavy metals, suggesting its potential application in the bioremediation of industrial pollutants. These findings, highlight E. profundum as a promising candidate for future biotechnological applications in environmental remediation.