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Browsing MS by Author "ABDUL SAMAD"

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    Fabrication of novel vildagliptin-loaded zinc oxide nanoparticles for anti-diabetic activity
    (UMT, Lhr, 2023) ABDUL SAMAD
    The aim of the current study was to synthesize vildagliptin loaded ZnO nanoparticles for enhanced efficacy in terms of increased retention time, minimize side effects and increased hypoglycemic effects. Diabetes mellitus is the fastly prevailing disease throughout the world. Vildagliptin is the standard drug which is used for the treatment of diabetes. But the drug has certain limitations associated with it which includes short half-life and high dosage frequency. Now a days, Nanotechnology has revolutionized in every field of life. In medicine, nanoparticles are used as safe drug carriers, as nano-medicines with increased bioavailability, in tissue engineering and in diagnosis of diseases. Various nanoparticles like ZnO nanoparticles itself have medicinal applications. In this work, ZnO nanoparticles were synthesized by precipitation method and then drug vildagliptin was loaded on them by batch adsorption method. Ultraviolet Visible spectroscopy, Fourier transform Infra-red spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), High performance liquid chromatography (HPLC) and Energy Dispersive Xray (EDX) analysis were performed for the characterization of synthesized Vildagliptin loaded ZnO nanoparticles. The morphology of the synthesized drug loaded nano-material was analyzed by SEM which shows the formation of product with flaky texture. The crystallinity of drug loaded nano-materials was analyzed by XRD which gave the average crystalline size of 21.94 nm. EDX analysis detected the presence of Carbon, Oxygen, Zinc and Nitrogen in the product. The drug loading efficiency was determined by using HPLC method. The maximum adsorption efficiency of 58% was obtained. Further, In vitro anti-diabetic activity was assayed by determining the α-amylase and DPP IV inhibition activity of the product formed. The formulation gave maximum inhibition of 82% and 94% of α-amylase and DPP IV respectively at concentration of 1000 µg/ml. The inhibition of α-amylase can be attributed to synergistic effect of ZnO nanoparticles and vildagliptin.