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Browsing School of Science (SSC) by Author "Amna Tabassum"

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    Compositional analysis of withania coagulans for its bioactive compounds
    (University of Management and Technology Lahore, 2017) Amna Tabassum
    Many plants have been recounted to have therapeutic uses to treat and cure several human health diseases and mortalities. Withania coagulans (W. coagulans) is one of these medicinal plants which is known to have phytochemical properties and pharmacognostic effects. This plant is usually notorious as "Vegetable rennet” or “Indian cheese maker” because of milk coagulating properties due to enzyme Withanin. Phytochemical screening exposes that plant contains steroids, alkaloids, tannins, flavonoids, saponins and phenolic compounds. The Total phenolic content assay (TPC) and DPPH method were demonstrated to study antioxidant study of methanolic, aqueous and n-hexane extract of W. coagulans. The DPPH activity outcomes showed that aqueous extract shows highest concentration of the phenolic compounds as compared to n-hexane and methanolic extracts. The TPC assay represented that maximum absorbance is gained by the aqueous extract (4.2587), which was almost double than the methanolic extract (1.9219) and four times the n-hexane extract (0.9206). Antibacterial activity of all the extracts of W. coagulans was executed against the four different pathogens using the test control Amoxycillin by agar well diffusion method. The methanolic sample of concentration 100mg/ml shows zone of inhibition (15mm) which is maximum against the Pseudomonas aeruginosa. The n-hexane extract of concentration 150mg/ml shows great zone (13mm) against the Streptococcus mutans. The aqueous extract of concentration 50mg/ml shows (13mm) as highest zone of inhibition for the Escherichia coli. Results suggested W. coagulans has strong potential to fascinate the scientific community by its medicinal presentations and can be reflected as one of the important medicinal plant.