Cloning of fused gene of Thymosin alpha-1-p28 domain of Azurin in E. Coli
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Date
2020
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UMT Lahore
Abstract
Thymosin alpha 1 is a hormone consists of 28 amino acid and play role in treatment of diseases because of its immune stimulation, anti-viral and anti-cancer properties. p28 is a peptide derived from bacterial azurin protein and have anti-tumor activity. Tī-1 and p28 are both fused with the help of GGGGS linker. The objectives are to clone the fusion protein in E. coli DH5ī cells. TA cloning is specifically used for the process cloning and other steps of cloning have been followed by using kit method and plasmid pTZ57R/T was used. The fusion protein expects to be a strong candidate for cancer therapy because it is not only beneficial in stimulating immune response but it also targets tumor cells and block its way to escape from immune surveillance.