Isolation and growth optimization of chromium resistance bacteria from wastewater
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Date
2023-02-03
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UMT Lahore
Abstract
The current study was aimed at the isolation of bacteria that is resistant to Chromium from industrial wastewater. As industries are increasing day by day, different environmental risks associated with them are also increasing. The major environmental risk associated with industries is the discharge of effluents containing heavy metals. Cr (VI) is one of the most toxic from of chromium and contamination of this metal poses a threat to the environment. In this study strains of different bacteria were isolated from the dye industry effluents. A total of 6-gram positive heavy metals resistant strains were isolated. By biochemical characterization different chromium resistant strains were identified. The bio sorption capacity of these strains for chromium was conducted at a concentration of 0.25mM. At different concentrations of chromium, all the strains showed different MIC and growth range which indicates that all the strains have different Cr resistance. Among all the selected bacterial strains MC3 and MC4 showed maximum chromium reduction ability in a short period of time. At MIC of 6mM, MC3 showed an optical density of 0.2 at a wavelength of 600nm while MC4 showed an optical density of 0.23 at 600nm. The strain MC3 reduced 1.6 to 0.4 of Cr (VI) at an OD of 540nm in a four- hour time period while the strain MC4 reduced Cr (IV) from 1.05 to 0.42 at an OD of 540nm in a four-hour time period. The Cr (VI) reduction ability of these strains showed that the strains MC3 and MC4 can probably be used for bioremediation of Chromium.