Isolation and characterization of culturable extremophile from potential areas of pakistan.
No Thumbnail Available
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UMT Lahore
Abstract
The aim of this study was the isolation and characterization of extremophilic bacteria from the hot springs of Hunza from Gilgit-Baltistan, Pakistan. One microbe was isolated which showed optimum growth at 50°C at pH 7.0± 0.2. Antibiotic sensitivity of the microbes was tested against various antibiotics which included azithromycin, levofloxacin, lincomycin, ciprofloxacin and erythromycin. The microorganism showed sensitivity against all the above mentioned antibiotics. Furthermore, growth curve was checked using the UV spectrophotometer at absorbance of 600nm against time. The bacterial isolates were also tested for extracellular enzyme plate assay for amylase and protease, which exhibited positive and weak activity, respectively. Different biochemical tests including catalase, oxidase, nitrate reduction, gelatinase etc. were performed along with fermentation of different sugars and amino acids. When the biochemical characterization of the sample was performed, it revealed that it was Bacillus stearothermophilus. The study confirmed that the bacteria was a true thermophile which can provide a good source for biotechnological purposes. This microbe can be a potential source to get industrially important thermostable enzymes. The indigenous product of such enzymes can contribute in national development of the country.