Isolation, characterization and efficacy of phage mj2 against biofilm forming multi-drug resistant enterobacter cloacae

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dc.contributor.authorMohsin Jamal
dc.contributor.authorSaadia Andleeb
dc.contributor.authorFazal Jalil
dc.contributor.authorMuhammad Imran
dc.contributor.authorMuhammad Asif Nawaz
dc.contributor.authorTahir Hussain
dc.contributor.authorMuhammad Ali
dc.contributor.authorSadeeq ur Rahman
dc.contributor.authorRajanna Das, Chythanya
dc.date.accessioned2019-07-09T11:48:51Z
dc.date.available2019-07-09T11:48:51Z
dc.date.issued2018
dc.description.abstractBiofilm is involved in a variety of infections, playing a critical role in the chronicity of infections. Enterobacter cloacae is a biofilm-formingandmulti-drug-resistant(MDR)nosocomialpathogenleadingtosignificantmorbidityandmortality.Thisstudy aimed at isolation of a bacteriophage against MDR clinical strain of E. cloacae and its efficacy against bacterial planktonic cells andbiofilm.AbacteriophageMJ2wassuccessfullyisolatedfromwastewaterandwascharacterized.Thephageexhibitedawide range of thermal and pH stability and demonstrated considerable adsorption to host bacteria in the presence of CaCl2 or MgCl2. Transmission electron microscopy (TEM) showed MJ2 head as approximately 62 and 54 nm width and length, respectively. It had a short non-contractile tail and was characterized as a member of the family Podoviridae [order Caudovirales]. The phage MJ2wasfoundtopossess11structuralproteins(12–150kDa)andadouble-strandedDNA genomewith anapproximate sizeof 40kb.Thelog-phasegrowthofE.cloacaebothinbiofilmandsuspensionwassignificantlyreducedbythephage.TheE.cloacae biofilm was formed under different conditions to evaluate the efficacy of MJ2 phage. Variable reduction pattern of E. cloacae biofilmwasobservedwhiletreatingitfor4hwithMJ2,i.e.,biofilmunderstaticconditions.Therenewedmediawithintervalsof 24, 72, and 120 h showed biomass decline of 2.8-, 3-, and 3.5-log, respectively. Whereas, the bacterial biofilm formed with dynamic conditions with refreshing media after 24, 72, and 120 h demonstrated decline in growth at 2.5-, 2.6-, and 3.3-log, respectively. It was, therefore, concluded that phage MJ2 possessed considerable inhibitory effects on MDR E. cloacae both in planktonic and biofilm forms.en_US
dc.identifier.citationJamal, M., Andleeb, S., Jalil, F., Imran, M., Nawaz, M. A., Hussain, T., ... & Das, C. R. (2019). Isolation, characterization and efficacy of phage MJ2 against biofilm forming multi-drug resistant Enterobacter cloacae. Folia microbiologica, 64(1), 101-111.en_US
dc.identifier.urihttps://escholar.umt.edu.pk/handle/123456789/3754
dc.language.isoenen_US
dc.publisherFolia microbiologicaen_US
dc.titleIsolation, characterization and efficacy of phage mj2 against biofilm forming multi-drug resistant enterobacter cloacaeen_US
dc.typeArticleen_US
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