Synthesis of anti IL-6 polyclonal antibody and its characteristics
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Date
2023-10-03
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UMT Lahore
Abstract
We present the synthesis and characterization of a polyclonal antibody targeting Interleukin-6 (IL-6), a key cytokine involved in various inflammatory and immune responses. The research involved the development of a novel polyclonal antibody and its subsequent evaluation in a murine model. The primary objective was to establish a reliable and specific antibody against IL-6, which is crucial for understanding its role in various physiological and pathological processes. The synthesis of the anti-IL-6 polyclonal antibody involved immunization of mice with recombinant IL-6, followed by serum collection and antibody purification. The resulting antibody was characterized through immuno dot blot and enzyme-linked immunosorbent assay (ELISA) techniques to confirm its specificity and affinity towards IL-6. Our findings reveal that the synthesized polyclonal antibody effectively recognizes and binds to IL-6 in murine samples, demonstrating its specificity and suitability for further research and diagnostic applications. This research contributes to the development of valuable tools for studying IL-6-related mechanisms in various biological contexts, ultimately enhancing our understanding of its role in health and disease. Additionally, the methodology and results presented in this thesis offer a foundation for future investigations involving IL-6 and related cytokines.