Isolation and characterization of antibiotic producing bacteria from the salt range of Kallar Kahar (Pakistan)
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Date
2019
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UMT Lahore
Abstract
The Streptomycetes are gram positive bacteria with a filamentous form that present in wide variety of soil including water and plants. Important characteristic of Streptomycetes is the ability to produce secondary metabolites such as antibiotics. The need to produce new antibiotics is to overcome the resistance in pathogens. Streptomyces produce over two-thirds of the clinically useful antibiotics of natural origin (e.g., neomycin and chloramphenicol). A culture collection of 8 Streptomyces strains was isolated from saline soil of Kalar Kahar (Punjab, Pakistan) using stringent methods and was screened biologically and chemically to find their antibacterial activity for the production of antibiotics. In biological screening the extracts obtained from the broth of selected strains were analyzed for their activity against a set of test organisms, including Gram positive, Gram-negative bacteria using agar plug and disk diffusion bioassay methods. The results depicted an impressive antibacterial activity were produced by these strains. Natural sources are abundantly present in nature that is why they got more importance in the discovery of new antibiotics. In the present era, need of new antibiotics discovery has got much importance due to the rapid development of resistance in the previously present antibiotics. Because of increasing microbial resistance to general antibiotics and inability to control infectious disease has given a call for continuous search of novel antibiotics in all over the world.