Expression of recombinant azurin domain from E.Coli
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Date
2019
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UMT Lahore
Abstract
Azurin is an anticancer protein, which is produced by Pseudomonas aeruginosa. It is also called cupredoxin (copper containing protein) having molecular mass 14kDa.It is a water-soluble protein.Whole Azurin domain is 128 amino acid long that helps in electron transport chain during denitrification process. Its p28 domain (molecular mass of 4kDa) is specifically involved in the anticancer activity. p28 due to its small size can be effective in treating cancer. This peptide directly targets on the cancerous cells without damaging any normal cells. This study involves the optimization of IPTG induction, time optimization in LB media, effect of temperature on bacterial growth and optimization of LB,YP and TB media. The results exhibit that the best concentration for the maximum production of transformed BL21 DH3 cells was at 1mM. Best time for the maximum production of transformed cells in LB media was 5 hours and maximum production of cells at different temperatures in TB, YP and LB media were maximum at 37°C. Optimization in three media exhibit that TB media showed maximum growth of transformed cells as compared to the other media.