Production of 1, 4-β-D Glucanase from bacterial co-culture using solid-state fermentation
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Date
2019
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UMT Lahore
Abstract
ellulase or 1,4-β-D-glucan 4-glucanohydrolase is an enzyme that cause the hydrolysis of cellulose or 1, 4-β linkages into simple glucose monomers. It is used worldwide because of its extensive role in food, wine, textile, laundry, pulp and paper industry, animal feed, agriculture, biomass, and refining industries. Cellulases form plants and animals are more expensive and cannot be readily used in industries so microbial cellulases are preferred over them because they can grow on environment friendly and cheap substrates. The present study deals with the isolation of cellulolytic bacterial strains and cellulase production from bacterial co-culture. Seven different bacterial strains were isolated from the soil, screened on cellulose agar media and further tested for their compatibility. Secondary screening was done for the bio-synthesis of enzyme using Solid state fermentation (SSF). Out of 10 bacterial co-cultures, co-culture no. 3 shown maximum yield of cellulase (514 U/ml) using 10g of solid substrate. The strains of co-culture no. 3 were further identified using morphological and biochemical tests. It was observed that both strains of bacterial co-culture no. 3 were gram-negative, strain 1 was rounded and strain 2 was rod-shaped. Both strains shown positive results for catalase, methyl red and endospore staining tests, but for voges proskauer strain 1 gave positive test. From this study, it was revealed that bacterial co-culture no.3 can be the high potential co-culture for maximum cellulase production on large scale.