Isolaion and optimization of efficient bacteria during thermophillic phase of municipal solid waste composting
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Date
2020
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UMT Lahore
Abstract
Composting is biological decomposition of organic waste into more stable humus like compound carried by microbial population. The complex organic matter into simpler form by the help of microbial activity of bacteria and fungi. Different physical and chemical parameter like pH, moisture content of substrate, oxygen concentration, carbon-nitrogen ratio, particle size play important factors in supporting stability of the final product. Thermophilic stage is the most active stage of composting starts by replacing microphillic flora and continues until 62℃ is reached. The optimum temperature for thermophilic stage is 35 to 55 °C. Thermophiles plays an important role and have a huge diversity, thermophilic phase was found to be dominant by Bacillus. On the other hand degree of humification and maturation process in composting is improved by microbial inoculants. This study is designed to isolate and identify most efficient degrading bacteria at thermophilic stage of composting. Growth of strain ‘X’ on different carbohydrates media and blood agar media was observed and evaluated. Optimization of Bacterial ‘X’ strain at different temperature and pH was also evaluated by spectrophotometry and electrical impedance. The most suitable temperature for better growth of bacteria ‘X’ was 450C and 5.5 pH.