Isolation and characterization of bacteria from soil samples and their role in plant growth promotion
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Date
2023-08-31
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UMT Lahore
Abstract
Soil enormous microbial diversity influences soil fertility and crop health through nutrient recycling and organic matter degradation. Our study aimed to 1) Isolate and purify bacteria from rhizosphere soil samples, 2) Morphological, biochemical characterization, and identification of isolated bacteria and 3) Determining plant growth promoting capability of identified bacterial isolates. Seven bacteria, three from mint soil and four from lettuce rhizospheric soil, were isolated, purified, and characterized. Gram staining, endospore staining, and acid fast staining performed along with ten different biochemical tests for bacterial identification. Plant growth promoting potential of isolates was determine by their growth on Pikovskaya’s agar medium and by plant microbe interaction experiment. Isolate Mnt1 showed phosphate solubilization ability. Isolate Mnt1 and Ltc4 showed the maximum increase in zea mays plant growth compared to the control and other isolates application. Based on the results, isolate 1M has plant growth promoting potential that can be utilized to enhance crop yield at commercial scale. It can be used to produce effective biofertilizers of economic importance. Isolate Mnt1 showed increased plant width by 80% and Ltc4 showed 44% increased weight. Moreover, Isolate Mnt2 showed negative impact and reduced plant growth, reduced plant’s weight by 53%.