Phenotypic characterization of MBL enzymes in urinary tract infection causing pathogens
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Date
2023-09-25
Journal Title
Journal ISSN
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Publisher
UMT Lahore
Abstract
Urinary tract infections (UTIs) are a common bacterial infection. UTIs are one of the most frequent bacterial infections in the entire world, affecting people of all ages and genders. Epidemiological data regarding UTIs can vary depending on the region, population demographics, healthcare access, and reporting practices. Metallo-β-lactamase is an enzyme that is resistant towards most of the antibiotics particularly among gram negative bacteria. In the current research study, the glycerol stocks of bacterial isolates were obtained from Life Science Laboratory, University of management and technology, Lahore and the culture was refreshed and screened on agar plates for MBL production. The antimicrobial resistivity was detected by using disc diffusion methods against meropenem in presence or absence of EDTA. MBL producers was identified and differentiated through biochemical testing. Furthermore, for the purpose of statistical analysis Graph pad prism software was used in this study which Chi-square test. In the current study, the species prevalent in the provided bacterial isolates of the suspected UTI patients were Klebsiella spp. (100%). Out of the total bacterial isolates (n=25), 3 were confirmed to be MBL producers. Percentages of MBL producing isolates were lower in males (8.33%) as compared to females (15.3%) and distribution was statistically non-significant (P=0.587).