Comparison of three different DNA extraction methods for betel quid spits and gutka spits collected from ten different surfaces
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Date
2020
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UMT Lahore
Abstract
Betel quid and Gutka chewing is habit of a mass population mainly consist of Asian countries and is also spread to other world through immigrants. Chewing of Betel quid and Gutka induce some chemical changes in the saliva of consumers due to the presence of a number of carcinogenic and other substances present in them which cause inhibition in the isolation of DNA from their chewer’s saliva. Saliva is an important examined biological fluid in forensic analysis. It is a significant evidence that can help to investigate a rape case, assault and homicide cases. This study was designed to make an evaluation and compare three manual methods consisting of Phenol Chloroform isoamyl alcohol (PCI) test, Trehalose test and Alkaline lysis test for DNA isolation from Betel quid and Gutka samples collected from ten different surfaces. The aim of this study was to find a most suitable method for DNA isolation to face these kind of challenging samples in the field of Forensic science. The surfaces which were supposed in this study were more susceptible as a part of crime scene where evidential saliva might be found. soil, marble, glass, cloth, wood, plastic, cardboard, paper, metal and plant material were the surfaces selected in this study. The isolated DNA was checked for concentration and purity through spectrophotometer method in order to evaluate the most favorable method for DNA isolation. In this study Phenol Chloroform Isoamyl Alcohol was found to be the most favorable and efficient method from two other methods for both Betel quid and Gutka spit samples. However, it was observed in this study that it is difficult to recover DNA from these kind of Forensic samples.