Screening of Ranunculus sceleratus for enzyme inhibition, antibacterial and antioxidant activities
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
A Journal of the Bangladesh Pharmacological Society (BDPS)
Abstract
Enzyme inhibition potential of various fractions of Ranunculus sceleratus was
checked against α-glucosidase, butyrylcholinesterase, acetylcholinesterase and
lipoxygenase enzymes. n-Butanol fraction showed very good activity (77.5 ±
1.0% inhibition at 0.1 mg/mL) against α-glucosidase. Its IC50 value was 35.7 ±
1.0 μg/mL comparable to quercetin (IC50 value 16.5 ± 0.4 μg/mL). Antibacterial
activity was checked against five bacterial strains by 96-wells microplate
assay using ciprofloxacin, a standard antibiotic. Chloroform, ethyl acetate, nbutanol
and aqueous fractions showed excellent activity against Pseudomonas
aeruginosa, (MIC at 7.1, 7.8, 5.6 and 5.3 respectively), which is greater than
standard antibiotic ciprofloxacin (MIC 10.0). The antioxidant potential of all
the fractions was evaluated. Ethyl acetate soluble fraction exhibited highest
percent inhibition of DPPH radical as compared to other fractions. It showed
80.9 ± 1.2% inhibition of DPPH radical at a concentration of 30 μg/mL. These
results suggest that R. sceleratus is a valuable herb, which inhibits the oxidative
stress mechanism that lead to degenerative diseases.
Description
Keywords
Antibacterial, Antioxidant, Enzyme Inhibition, Ranunculus Sceleratus
Citation
Shahid, S., Riaz, T., & Asghar, M. N. (2015). Screening of Ranunculus sceleratus for enzyme inhibition, antibacterial and antioxidant activities. Bangladesh Journal of Pharmacology, 10(2), 436-442.