Extraction and purification of lipase from aspergillus niger
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Date
2018
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UMT Lahore
Abstract
Lipase belongs to the serine class and catalyzes the hydrolysis of triglycerides to fatty acid and glycerol. Lipase is water soluble enzyme. Lipases have great importance in industry. Lipase used in different industries such as food processing, pharmaceutical, detergent, leather, cosmetics. Several plants, animals, fungi and bacteria are recognized to produce lipases. One of the major applications of enzymes is in durable detergents for household laundry. Lipase show different positional specificities and can catalyze different reactions like esterification, interesterification, and hydrolysis.
Isolate the lipase from Aspergillusniger. Purification step was performed by ammonium sulfate precipitation and dialysis. Titrimetric method was used to estimate the specific activity of the enzyme. Bradford assay method was used to estimate the concentration of protein. SDS-PAGE was used to check the molecular weight of enzyme. The maximum extracellular lipase was purified 2.74 folds with an overall yield of 0.65% through purification procedure of ammonium sulfate precipitation. Maximum lipase activity of 0.23 U/ml and specific activity 21.9 U/ml was observed using olive oil as substrate. Protein estimation showed the concentration of enzyme present in the sample that was 420 mg/ml. Molecular weight determination was done by SDS-PAGE bands were not visualized on the gel, due to low concentration of protein,