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Browsing Faculty Research by Subject "A-glucosidase"

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    In vitro assessment of antibacterial, enzyme inhibition and oxidative stress activities of various fractions of ranunculus sceleratus
    (2013) Sammia Shahid
    The aims of the present investigation were to assess the enzyme inhibition, antibacterial and antioxidant activities of various fractions of. Ranunculus sceleratus Linn. The methanolic extract of plant was partitioned sequentially with solvents of increasing polarity. Enzyme inhibition studies were done against four enzymes i.e. α-glucosidase, butyrylcholinesterase, acetylcholinesterase and lipoxygenase. It was observed from the results that n-butanol fraction possessed very good activity (77.49±0.94 % inhibition at 0.1 mg/ml) against α-glucosidase, as compared with quercetin, a reference standard drug. Its IC50 value was calculated as 35.69±0.93 μg/ml as compared to quercetin (IC50 value 16.47±0.39 μg/ml). Antibacterial activity was checked against five bacterial strains by 96-wells microplate assay using ciprofloxacin, a standard antibiotic, as positive control. Chloroform, ethyl acetate, n-butanol and aqueous fractions showed excellent activity against Pseudomonas aeruginosa, (MIC at 7.09, 7.82, 5.62 and 5.26 respectively) which is greater then standard antibiotic ciprofloxacin (MIC 10.03). Ethyl acetate n-butanol and aqueous fraction also showed good activity against Escherichia coli (MIC at 10.56, 10.88 and 10.74 respectively). The antioxidant potential of all the fractions was evaluated by different methods. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 80.9±1.23% inhibition of DPPH radical at a concentration of 30 μg/ml. The IC50 value of this fraction was 44.07±0.76 μg/ml, relative to ascorbic acid, having IC50 58.90± 1.76 μg/ml. It also showed highest total antioxidant activity (1.04±0.03) as well as highest FRAP value (238.5±1.12 TE μM/ml), highest total phenolic contents (97.08±1.03 GAE mg/g) and highest value of inhibition of lipid peroxidation (53.70±1.59%) as compared to the other studied fractions. The results obtained from this study suggest that Ranunculus sceleratus is a valuable herb, which due to the presence of larger quantities of enzyme inhibition, antibacterial and antioxidant compounds inhibits the oxidative stress mechanism that lead to degenerative diseases.