(UNIVERSITY OF MANAGEMENT AND TECHNOLOGY, 2015) Maaz Asim
A simple, rapid and time saving method for the validation of Amoxillin Trihydrate in capsule dosage form has been developed. Recovery studies was achieved by HPLC for this chromatographic separation was achieved by phenomenex [250x4.6 mm with octadecylisily silica gel for chromatograph (5μm)] column on binary mode and mobile phase (A) contain 6.8g of potassium dihydrogen phosphate buffer (adjust to pH 5.0 by sodium hydroxide solution) containing Acetonitrile with ratio of 99:1 and mobile phase (B) containing Buffer and Acetonitrile with ratio of 80:20. The flow rate was 1 ml/min with 8% concentration of mobile phase (B) and was monitored at 254 nm. Retention time was found to be near 7.1 for Amoxicillin Trihydrate. The regression value obtained was 0.9999 with 3.0011 Limit of Detection and 9.0943 Limit of Quantization. Residual solvents were also validated for AMT by using GC-FID and observed that there is no presence of any solvent in it. Other parameters like FTIR, pH, Optical rotation and Moisture contents were also performed for further analysis and all results were satisfactory and was meeting the require values. The projected methods are accurate, exact, selective and rapid for the estimation of Amoxicillin Trihydrate in pharmaceutical dosage forms.