Browsing by Author "USMAN ALI"

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    A BLOCKCHAIN-BASED FRAMEWORK FOR DISTRIBUTED DEVOPS
    (UMT, Lahore, 2023) USMAN ALI
    Distributed DevOps is a software development methodology that aims to integrate the work of development and operations teams without being bound by geographical constraints. This methodology excels in enhancing collaboration and the speed of software development. However, it does suffer from a lack of security, transparency, and traceability, which can result in project delays, a lack of trust between stakeholders, and even project failure. This paper addresses these issues of Distributed DevOps by implementing blockchain technology. In this paper, we propose a framework that utilizes the benefits of blockchain technology to eliminate the shortcomings of Distributed DevOps. We present performance results that show the effectiveness of our framework in a real-world scenario, highlighting its ability to improve transparency, traceability, and the security of the DevOps pipeline. In conclusion, our research contributes to the growing literature on the intersection of blockchain and DevOps. It provides a practical framework for organizations looking to leverage blockchain technology to improve their development processes.
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    Evaluation and quantification of genomic DNA from fresh and old blood samples
    (UMT Lahore, 2025-09-02) USMAN ALI
    The success of molecular biology experiments is also most significantly via the recovery of good-quality and adequate-quantity genomic DNA from biological samples. Compared to all the traditional sources, blood is most frequently more normal in preference because it is easily accessible and is found with nucleated cells of high density. Storage conditions, sample age, and extraction procedure lead to DNA damage. This research is to determine the quality and yield of genomic DNA recovered from 52 mammalian blood samples, fresh and old, based on modified salting-out and PCI protocols. DNA quality was confirmed by employing mainly agarose gel electrophoresis, for which clear, visible DNA bands were recorded for 14 samples, demonstrating the success of extraction by both modified protocols. Other than the purity and concentration test, the DNA samples were even passed through the NanoDrop 1000 spectrophotometer. Good purity and good concentration in good ranges for further use in some samples were recorded in new samples as well as in old samples. Protein or impurities of some other kinds were found in some extracts as well, primarily in old samples, which could be because of storage issues or degradation. Salting-out technique, however, was more improved, easier, and safer with harmless organic solvents, but with high-quality DNA for molecular research. Compared to that, the PCI method, although earlier thought to be highly efficient, was more accurate, along with the toxic nature, and hence not as perfect for bulk or repeated use. Finally, among the outcomes of the study are that both procedures can produce usable genomic DNA, albeit more so with the salting-out procedure, which is more convenient and even easier, especially for laboratory applications under high-throughput conditions. The study also determines the effect of sample aging on the quality of DNA and concludes on the necessity of the use of proper protocols of extraction in the estimation of efficiency, safety, and reproducibility of molecular genetic studies.