Browsing by Author "Shahid Mehmood"

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    Prediction of protein solubility in escherichia coli and experimental verification
    (UMT, Lhr, 2016) Shahid Mehmood
    Soluble protein in proper concentration is very important for different experimental studies. Solubility of protein can be estimated by the sequence of amino acids in protein. The solubility of protein is important for biophysical and structural development. To achieve the soluble protein in high concentration is a major challenge. The protein which are heterologous expressed are often insoluble and their solubilization is highly trial and error process with low success rate. Although very highly overexpression in inclusion body is some time desirable which result in clean protein. A new method is develop which will predict the solubility of protein on overexpression in E.coli. This method use four classifier named as Multilayer Perceptron, Decision Tree, Random Forest, Bayes Classifier. Theses classifier were trained for the prediction of recombinant protein solubility. Many features are used by this method such as canonical variable (CV),Surrounding hydrophobicity, Solubility index composition, Intrinsic aggregation propensity, Intrinsic Z-scores for aggregation, = tripeptide score, AI = aliphatic index, II= instability index, Fn= frequency of occurrence of Asn, Ft = frequency of occurrence of Thr, Fy= frequency of occurrence of Tyr. It is very simple and easy method for the prediction of recombinant protein solubility. To evaluate the validity of this method test is performed. For this purpose dataset consist of 1500 proteins, out of which 1000 are soluble and 500 are insoluble. Each classifier was trained for the prediction of 450 protein sequences. This method will predict the protein solubility with greater accuracy of about 95.9%. The accuracy of this method is also compared with the previous work or methods. Results shows that this method has more accuracy and precision then other previous works.
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    (UMT.Lahore, 2004) Zia-Ur-Rehamn; Shahzad Ahmad; Shahid Mehmood
    Human requirements are unlimited. Human beings want to achieve those unlimited requirements in a limited time. As a consequence, they want to do all works as fast as possible with highest degree of reliability. This in turn requires some sort of automation (i.e. work with less human intervention). We have also tried to lessen the human interaction and find best solutions for the Websites as well as web projects creation. On biggest advantage of our Desktop application is that it provides unique platform for all the users so they can work more efficiently and effectively.