Browsing by Author "SHAHID YOUNIS"

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    Anticancer activity of ethyl acetate extract of tanacetum parthenium
    (UMT, Lhr, 2015) SHAHID YOUNIS
    The aim of research was to evaluated the proximate analysis, phytochemical screening, antibacterial activity, anticancer activity of the Tanacetum parthenum plant. The proximate analysis was used to characterize the plant on the basis of different percentage of different plants contents and so percentage of moisture, fiber, and ash, fat was determined. The phytochemical screening of plant indicated that chemical compound i.e. flavonoids, tannins, reducing sugar, starch, coumarins was indicated in it and due to the presence of different active chemical compounds it was used against bacteria. The plant extract has shown positive inhibition effect against bacteria and it restricts the growth of bacteria. The prepared solution of ethyl acetate and methanol were applied to check their effect on the growth of cancerous HeLa cell line. The neutral red dye assay was used to evaluated the percentage viability of the cells after treated with ethyl acetate and methanol extract. The treated cells were studied at 540 nm to check their absorbance. The 100% absorbance of the control group was compared with absorbance of treated experimental cancer cells. The decrease in absorbance means decrease in cancerous cell after treated with extracts. The concentration of acetate extract was found to be 128 folds dilution (0.004 µg) at which the number of cancer cells reduced to 50%. The half inhibitory concentration of the methanolic extract was greater than 128 folds dilution at which viability of the cells were reduced to 50% so, methanol extract is more potent inhibitor for cancerous activity of the cells.